海藻异枝麒麟菜多糖对纳米羟磷灰石晶体损伤小鼠血管平滑肌细胞的影响

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摘要目的探讨海藻异枝麒麟菜多糖（ESP）对纳米羟基磷灰石（nano-HAP）晶体诱导的小鼠血管平滑肌细胞（MOVAS）损伤的影响。方法将体外培养的血管平滑肌细胞分为空白对照组、模型组、实验组。空白对照组加入新鲜培养基，模型组给nano-HAP刺激24 h，实验组用不同浓度（50、150、450 μg/mL）的ESP处理模型组细胞。采用MTT法检测细胞的活力，乳酸脱氢酶（LDH）试剂盒检测细胞LDH释放量，采用苏木精-伊红染色观察细胞形态。结果与空白对照组比较，模型组细胞活力明显下降（P ＜ 0.05），LDH释放量明显升高（P ＜ 0.05）；与模型组比较，实验组细胞活力随着加入ESP浓度的增加而升高，LDH释放量随着ESP浓度的增加而逐渐下降，差异均有统计学意义（均P ＜ 0.05）。结论 ESP可减轻nano-HAP晶体对血管平滑肌细胞膜的损伤。

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	韩锦1 姚智会2 马晓桃1 桂保松1

关键词 ：血管钙化, 羟基磷灰石, 海藻异枝麒麟菜, 降解多糖

Abstract：Objective To investigate the effect of algae eucheuma striatum polysaccharide (ESP) on mouse vascular smooth muscle cells injured (MOVAS) induced by nano-hydroxyapatite crystals (nano-HAP). Methods Cultured vascular smooth muscle cells were divided into the blank control group, the model group and the experimental group. Fresh medium was added to the control group, the model group was stimulated by nano-HAP for 24 h. The cells in the experimental group were treated with different concentrations (50, 150, 450 μg/mL) of ESP. The activity of cells was determined by MTT assay and LDH release by LDH kit, cell morphology was observed by hematoxylin-eosin staining. Results Compared with the blank control group, cell viability in the model group decreased significantly (P ＜ 0.05), LDH release was significantly increased (P ＜ 0.05). Compared with the model group, the activity of cells in the experimental group increased with the increase of ESP concentration, and the release of LDH decreased with the increase of ESP concentration, with statistically significant differences (all P ＜ 0.05). Conclusion ESP can alleviate the damage of nano-HAP crystal to the membrane of vascular smooth muscle.

Key words： Vascular calcification Hydroxyapatite Algae eucheuma striatum Degraded polysaccharide

基金资助:国家自然科学基金资助项目（81570673）。

通讯作者: 桂保松（1957.4-），男，主任医师；研究方向：血液净化抗凝技术和枸橼酸抗血管钙化及尿结石系列研究。

作者简介: 韩锦（1984.11-），女，硕士；研究方向：慢性肾脏病及血管钙化。

引用本文:

韩锦1 姚智会2 马晓桃1 桂保松1. 海藻异枝麒麟菜多糖对纳米羟磷灰石晶体损伤小鼠血管平滑肌细胞的影响[J]. 中国医药导报, 2019, 16(33): 4-6,35.
HAN Jin1 YAO Zhihui2 MA Xiaotao1 GUI Baosong1. Effect of algae eucheuma striatum polysaccharide on mouse vascular smooth muscle cells injured by nano-hydroxyapatite crystals. 中国医药导报, 2019, 16(33): 4-6,35.

链接本文:

http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I33/4

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