载脂蛋白E基因多态性检测在脑梗死中的意义

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摘要目的探讨载脂蛋白E（ApoE）基因多态性与脑梗死的发生发展的相关性。方法选择2015年1月～2017年3月在佛山市第一人民医院（以下简称“我院”）神经内科住院的348例脑梗死患者作为病例组，选择同期我院体检科352名健康人群作为对照组，采用基因芯片方法进行ApoE基因型检测，比较两组ApoE基因型及表型的构成差异，及ApoE不同表型患者血脂水平。结果病例组和对照组ApoE基因型均以ε3/3频率最高，等位基因频率以ε3最高；病例组ε2/3和ε3/3基因型均低于对照组，差异有统计学意义（P < 0.05），ε3/4显著高于对照组，差异有高度统计学意义（P < 0.01）；病例组ε2等位基因频率显著低于对照组，差异有统计学意义（P < 0.05），而ε4基因频率显著高于对照组，差异有高度统计学意义（P < 0.01）；病例组中不同ApoE表型患者血脂水平相比，E2型患者总胆固醇及低密度脂蛋白胆固醇水平最低，差异有统计学意义（P < 0.05）。结论 ApoE基因多态性在脑梗死的发生发展中起一定作用。

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	李美珠朱嫦琳陈斌鸿李炜煊

关键词 ：载脂蛋白E, 脑梗死, 基因多态性, 血脂

Abstract：Objective To investigate the correlation between the gene polymorphism of apolipoprotein E (ApoE) and the development of cerebral infarction. Methods The case group of 348 patients with cerebral infarction in Neurology Department of the First People′s Hospital of Foshan ("our hospital" for short) from January 2015 to March 2017, and control group of 352 healthy people at the same time in our hospital were selected. Both groups were used the gene chip approach for the ApoE genotype tested. ApoE genotype and phenotype between the two groups, and the serum lipid levels of patients with different ApoE gene phenotypes were compared. Results The highest genotype frequency of ApoE was ε3/3 and the highest allele frequency was ε3 in the two groups. The ε2/3 and ε3/3 genotypes in the case group were significantly lower than the control group, the differences were statistically significant (P < 0.05); and the ε3/4 was significantly higher than the control group, the difference was highly statistically significant (P < 0.01). The frequency of ε2 allele in case group was significantly lower than the control group, the difference was statistically significant (P < 0.05), while the ε4 was significantly higher than the control group, the difference was highly statistically significant (P < 0.01). Compared with the levels of blood lipids in different ApoE phenotypes patients of the case group, the levels of total cholesterol and low density lipoprotein cholesterin were the lowest in E2 genotype patients, the differences were statistically significant (P < 0.05). Conclusion The ApoE gene polymorphism plays an important role in the occurrence and development of cerebral infarction.

Key words： Apolipoprotein E Cerebral infarction Gene polymorphism Serum lipid

通讯作者: 李炜煊（1960.12-），男，主任技师；研究方向：实验室管理学。

作者简介: 李美珠（1966.7-），女，主任技师；研究方向：生物化学、免疫学、分子生物学。

引用本文:

李美珠朱嫦琳陈斌鸿李炜煊 . 载脂蛋白E基因多态性检测在脑梗死中的意义[J]. 中国医药导报, 2017, 14(34): 110-113.
LI Meizhu ZHU Changlin CHEN Binhong LI Weixuan. The significance of apolipoprotein E gene polymorphism in cerebral infarction. 中国医药导报, 2017, 14(34): 110-113.

链接本文:

http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2017/V14/I34/110

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