AFP基因多态性与肝细胞癌易感性的相关性研究

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摘要目的探讨甲胎蛋白（AFP）基因的单核苷酸多态性及血清AFP水平与原发性肝细胞癌（HCC）发病风险的关系。方法纳入2017年7～12月在广西医科大学附属肿瘤医院的206例患者为研究对象，其中HCC患者118例（HCC组），非肿瘤患者88例（对照组）。采用化学发光免疫分析法检测血清AFP水平，应用SNaPshot技术对AFP基因rs4024和rs737241位点多态性进行分型。结果 rs7372412位点的基因型和等位基因在HCC组和对照组中的分布频率差异有统计学意义（P ＜ 0.05）；携带GA或AA基因型的个体患HCC的风险是携带GG基因型的个体的0.543倍（P = 0.037，OR = 0.543，95%CI：0.306～0.965）；携带A等位基因的个体患HCC的风险是携带G等位基因个体的0.573倍（P = 0.008，OR = 0.573，95%CI：0.379～0.865）。血清AFP水平与年龄、巴塞罗那（BCLC）分期和肝癌病理分级（EDSON）相关（均P ＜ 0.05），且年龄越大，血清AFP水平越低（P = 0.001，OR = 0.172，95%CI：0.059～0.501）；BCLC分期越高，血清AFP越高（P = 0.000，OR = 4.420，95%CI：2.003～9.752）；EDSON分级越高，血清AFP越高（P = 0.033，OR = 2.335，95%CI：1.068～5.102）。结论 AFP基因rs737241位点多态性可能与HCC的遗传易感性有关。血清AFP水平可能与年龄和HCC的发展及恶化相关。

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	黄玉亮吴君荣赵惠柳刘志民梁艺华黄昭东朱波▲

关键词 ：甲胎蛋白, 原发性肝细胞癌, 单核苷酸多态性, 易感性

Abstract：Objective To investigate the relationship between the single nucleotide polymorphisms of alpha fetal protein (AFP), serum AFP levels and the onset risk of primary hepatocellular carcinoma (HCC). Methods Two hundred and six patients in the Affiliated Tumor Hospital of Guangxi Medical University from July to December 2017 were enrolled, among which, 118 cases were HCC patients (HCC group), 88 cases were non-tumor patients (control group). The serum AFP levels were detected by chemiluminescence immunoassay, and the polymorphism of rs4024 and rs737241 of AFP gene was analyzed with SNaPshot technique. Results The frequency of genotype and allele at site rs737241 in the HCC group and control group had a significant difference (P ＜ 0.05). The risk of HCC in the subjects carrying GA or AA genotype was 0.543 times as big as the individuals carrying GG genotype (P = 0.037, OR = 0.543, 95%CI: 0.306-0.965). The onset risk of HCC in individuals carrying A allele was 0.573 times as big as those carrying G allele (P = 0.008, OR = 0.573, 95%CI: 0.379-0.865). The serum AFP levels were correlated with age, Barcelona clinic liver cancer (BCLC) stage and Edmondson-Steiner (EDSON) grade (P ＜ 0.05), the older the age, the lower the serum AFP levels (P = 0.001, OR = 0.172, 95%CI: 0.059-0.501); the higher the BCLC stage, the higher the serum AFP levels (P = 0.000, OR = 4.420, 95%CI: 2.003-9.752); the higher the EDSON grade, the higher the serum AFP levels (P = 0.033, OR = 2.335, 95%CI: 1.068-5.102). Conclusion The polymorphisms of rs737241 for AFP gene may be correlated with the genetic susceptibility of HCC. The serum AFP level may be correlated with the age, the development and deterioration of HCC.

Key words： Alpha fetal protein Primary hepatocellular carcinoma Single nucleotide polymorphism Susceptibility

基金资助:国家自然科学基金资助项目（81760530）；
广西自然科学基金资助项目（2012GXNSFAA053170、2012GXNS FBA198047）。

通讯作者: ▲通讯作者

引用本文:

黄玉亮吴君荣赵惠柳刘志民梁艺华黄昭东朱波▲. AFP基因多态性与肝细胞癌易感性的相关性研究[J]. 中国医药导报, 2019, 16(28): 25-29.
HUANG Yuliang WU Junrong ZHAO Huiliu LIU Zhimin LIANG Yihua HUANG Zhaodong ZHU Bo▲. Research on the correlation of AFP gene polymorphism with the susceptibility of hepatocellular carcinoma. 中国医药导报, 2019, 16(28): 25-29.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I28/25

[1] 姜楠，李述刚，闫贻忠，等.2005－2009年中国肿瘤登记地区肝癌疾病负担变化趋势分析[J].现代预防医学，2017， 44（19）：3483-3507.
[2] 余家华，李秋林，容敏华，等.2013年广西肿瘤登记地区恶性肿瘤发病和死亡分析[J].中国癌症防治杂志，2017， 1（9）：30-39.
[3] Suriapranata IM，Sudania WM，Tjong WY，et al. Alpha-fetoprotein gene polymorphisms and risk of HCC and cirrhosis [J]. Clin Chim Acta，2010，411（5/6）：351-358.
[4] Meng-Sen Li，Qiu-Ling Ma，Qian Chen，et al. Alpha-fetoprotein triggers hepatoma cells escaping from immune [J]. World J Gastroenterol，2005，11（17）：2564-2569.
[5] Zhang C，Chen X，Liu H，et al. Alpha fetoprotein mediates HBx induced carcinogenesis in the hepatocyte cytoplasm [J]. Int J Cancer，2015，137（8）：1818-1829.
[6] Li P，Wang SS，Liu H，et al. Elevated serum alpha fetoprotein levels promote pathological progression of hepatocellular carcinoma [J]. World J Gastroenterol，2011，41（17）：4563-4571.
[7] Liao X，Han C，Qin W，et al. PLCE1 polymorphisms and expression combined with serum AFP level predicts survival of HBV-related hepatocellular carcinoma patients after hepatectomy [J]. Oncotarget，2017，8（17）：29202-29219.
[8] Silva JP，Gorman RA，Berger NG，et al. The prognostic utility of baseline alpha-fetoprotein for hepatocellular carcinoma patients [J]. J Surg Oncol，2017，116（7）：831-840.
[9] Chen GG，Ho RL，Wong J，et al. Single nucleotide polymorphism in the promoter region of human alpha-fetoprotein（AFP）gene and its significance in hepatocellular carcinoma（HCC）[J]. Eur J Surg Oncol，2007，33（7）：882-886.
[10] 蒋明明.肝细胞癌和胆囊癌基因表达谱分析及肝癌相关基因单核苷酸多态性研究[D].上海：第二军医大学，2016.
[11] 莫国辉.广西地区原发性肝细胞癌患者甲胎蛋白基因多态性检测及其临床意义分析[D].南宁：广西医科大学，2012.
[12] Deshpande N，Chavan R，Bale G，et al. Hereditary Persistence of Alpha-Fetoprotein Is Associated with the -119G ＞ A Polymorphism in AFP Gene [J]. ACG Case Rep J，2017，4：e33.
[13] Jeon Y，Choi YS，Jang ES，et al. Persistent alpha-Fetoprotein Elevation in Healthy Adults and Mutational Analysis of alpha-Fetoprotein Promoter，Enhancer，and Silencer Regions [J]. Gut Liver，2017，11（1）：136-141.
[14] Debruyne EN，Delanghe JR. Diagnosing and monitoring hepatocellular carcinoma with alpha-fetoprotein：new aspects and applications [J]. Clin Chim Acta，2008，395（1/2）：19-26.
[15] 张平武，方林.GP73联合AFP-L3检测在低浓度甲胎蛋白肝癌患者诊断中的应用[J].肝脏，2018，23（9）：804-806.
[16] 张泽亮，王黎明，任龙飞，等.AFP通过激活STAT3的磷酸化促进肝癌细胞的增殖[J].中国肿瘤，2018，27（10）：796-800.
[17] 马鹏.肝细胞肝癌患者术前血清AFP浓度与肿瘤病理分级、临床分期及预后的相关性分析[D].新乡：新乡医学院，2018.
[18] 李广欣，王婧，曹邦伟，等.免疫检查点抑制剂治疗肝细胞癌的研究现状与进展[J].中国医院用药评价与分析，2018，18（4）：440-442，445.
[19] 丘海.血清AFP阳性胃癌的临床病理特征与预后分析[D].南宁：广西医科大学，2016.