Correlation analysis between mitochondrial DNA D-LOOP point mutation and long-term chronic hepatitis and hepatocellular carcinoma
LIU Yun1 LIU Daojie2 QIAO Luxin1 CHEN Dexi1▲
1.Beijing Youan Hospital, Capital Medical University, Beijing Institute of Hepatology, Beijing 100069, China;
2.Laboratory Medicine, Haidian Maternal ＆ Child Health Hospital of Beijing City, Beijing 100080, China
Abstract：Objective To analyze the changes in bases of mitochondrial DNA D-LOOP in various tissues by collecting specimens of hepatitis B virus (HBV)-associated liver cancer patients, and to explore the correlation between HBV-associated liver cancer and mitochondrial DNA D-LOOP. Methods Forty-six patients with chronic hepatitis B-related hepatocellular carcinoma (HCC) who were admitted to Beijing Youan Hospital, Capital Medical University from October 2008 to December 2019 were enrolled in the study. Thirty-one cases of cancerous tissues and corresponding adjacent tissues and peripheral blood, and 7 cases of adjacent tissues and corresponding peripheral blood, 5 cases of cancerous tissues and corresponding peripheral blood, 11 cases of cancer tissues and corresponding adjacent tissues. At the same time, 11 liver tissues from donors of liver transplantation patients and corresponding peripheral blood were selected as controls. The mitochondrial DNA of the specimen was extracted, primers and PCR reactions were designed to obtain the mitochondrial D-LOOP region, and the PCR product was sequenced for one generation. Results The results of one-generation sequencing data showed that long-term chronic HBV hepatitis caused a large number of single-base mutations in mitochondrial DNA D-LOOP region of inflammatory liver tissue and liver cancer, and the difference between liver cancer tissues and liver tissues was statistically significant (P < 0.05). Conclusion Long-term chronic HBV hepatitis can cause a large number of mitochondrial DNA D-LOOP region mutations in hepatitis tissues. Hepatoma tissue is different from normal liver tissue mutation mode, which has implications for the early diagnosis of liver cancer.
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2019, Vol. 16 
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