1.Department of Neurology, the First Affiliated Hospital of Harbin Medical University, Heilongjiang Province, Harbin 150001, China;
2.Department of Neurology, the Second Hospital of Qinhuangdao, Hebei Province, Qinhuangdao 066600, China;
3.Department of Neurology, the Second Affiliated Hospital of Harbin Medical University, Heilongjiang Province, Harbin 150086, China
Abstract：To investigate the expression of NgR and p-tau in senescence accelerated mouse P8 (SAMP8) was studied by using Nogo receptor (NgR) antagonist NEP1-40, explore the possible role of NgR. Methods Six months old senescence accelerated mouse-resistence 1 (SAMR1) male mice were the control group (group A); 3 months old SAMP8 mice (group B). Sham treatment group (group C) and treatment group (group N) of SAMP8 mice at 6 months of age, with 6 mice in each group. Mice in group C were intraperitoneally injected with 0.2 mL 0.9% normal saline, and mice in group N were intraperitoneally injected with 0.2 mL NEP1-40 for 14 days. Morris water maze test was used to monitor behavioral changes in mice before and after treatment, and immunohistochemical staining was used to detect changes in p-Tau and NgR expression in nerve cells. Results Compared with group A, the incubation periods of water maze test in group B, C and N were significantly prolonged, and the times of crossing platform were significantly reduced (P < 0.05). Compared with the first water maze experiment, the incubation period of group N was significantly shortened and the times of crossing platform were significantly increased (P ＜ 0.05). The NgR positive cell count and optical density of the hippocampus in group C were significantly increased compared with those in group A and group N (P ＜ 0.05). The deposition of p-Tau positive cells in group C was significantly reduced compared with that in group A and group N(P ＜ 0.05). Conclusion The learning and memory ability of SAMP8 mice decreased with the aging of the month, and NgR and p-Tau increased significantly in the hippocampus, which was consistent with the aging degree of the mice. NgR antagonist NEP1-40 may improve the learning and memory ability of mice by down-regulating NgR expression and reducing downstream p-Tau expression.
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2019, Vol. 16 
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