Expression of JAK2-STAT3 signaling pathway in lung tissues of rats with severe acute pancreatitis and its correlation with peripheral inflammatory factors
SHEN Yinfeng JIN Wenyin ZHANG Xia CHEN Qian
Department of Surgery Ⅳ, Affiliated Hospital of Hubei University of Traditional Chinese Medicine Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430070, China
Abstract：Objective To investigate expression of JAK2-STAT3 signaling pathway in lung tissues of rats with severe acute pancreatitis (SAP) and its correlation with peripheral inflammatory factors. Methods 5-month-old male Wistar rats (n = 240) were randomly divided into SAP group, SAP+R group, SAP+S group, SAP+R+S group and sham-operated group (SO group), 48 rats in each group. Abdominal aortic blood and lung tissues were collected 3, 6, 12 and 18 hours after model establishment in each group animals. Serum interleukin-6 (IL-6) and interleukin-10 (IL-10) were measured. The expression levels of JAK2 mRNA, STAT3 mRNA, p-JAK2 Protein and p-STAT3 protein in lung tissues were detected. Results The levels of serum IL-6 and IL-10 at 3, 6, 12 and 18 hours after model establishment were significantly different among the five groups (P < 0.05). There was significant difference in serum IL-10 level in SO group (P < 0.05). The serum levels of IL-6 and IL-10 in SAP group, SAP+R group, SAP+S group and SAP+R+S group increased gradually at 3 hours after model establishment, peaked at 12 hours after model establishment, and then decreased, there were statistically significant differences among different time points with in the group (P < 0.05). The expression levels of JAK2 mRNA, STAT3 mRNA, p-JAK2 protein and p-STAT3 protein in lung tissue of five groups at 3, 6, 12 and 18 hours after model establishment were significantly different (P < 0.05). The expression levels of JAK2 mRNA, STAT3 mRNA, p-JAK2 protein and p-STAT3 protein protein in lung tissues of five groups increased gradually at 3 hours after model establishment, peaked at 12 hours after model establishment, and then decreased, there were statistically significant differences among different time points within groups (P < 0.05). Conclusion Activation of JAK2/STAT3 pathway may be closely related to systemic inflammatory response in the course of SAP.
沈银峰 金文银 张霞 陈乾. JAK2-STAT3信号通路在重症急性胰腺炎大鼠肺组织中表达与外周炎性因子的相关性[J]. 中国医药导报, 2019, 16(26): 17-21.
SHEN Yinfeng JIN Wenyin ZHANG Xia CHEN Qian . Expression of JAK2-STAT3 signaling pathway in lung tissues of rats with severe acute pancreatitis and its correlation with peripheral inflammatory factors. 中国医药导报, 2019, 16(26): 17-21.
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2019, Vol. 16 
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