针刺对大鼠酒精戒断焦虑症的治疗效果及与CRH的关系

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摘要目的观察并探究针刺治疗大鼠酒精戒断焦虑症的作用及其机制。方法选取SPF级健康雄性SD大鼠54只，采用完全随机分组方法将其分为空白组、模型（酒精戒断焦虑症模型）组、三阴交组、足三里组、电针两穴（三阴交+足三里）组、针刺两穴（三阴交+足三里）组，每组9只。除空白组外，其余各组均连续腹腔注射酒精28 d制备酒精成瘾模型，撤酒精3 d制备酒精戒断焦虑症模型，撤酒精期间分别针刺相应穴位进行治疗，针刺3 d。采用高架十字迷宫（EPM）测其焦虑行为，酶联免疫吸附测定法（ELISA）检测海马中促肾上腺皮质激素释放激素（CRH）浓度，实时荧光定量PCR法检测海马中CRH mRNA的表达。结果与空白组比较，模型组进入开放臂次数比例和停留时间比例明显减少（P ＜ 0.05），海马中CRH浓度明显升高（P ＜ 0.01），CRH mRNA的表达显著升高（P ＜ 0.01）；与模型组比较，电针两穴组在EPM中进入开放臂次数比例、停留时间比例明显增加（P ＜ 0.05或P ＜ 0.01），针刺两穴组进入开放臂次数比例增加（P ＜ 0.05），电针两穴组和针刺两穴组海马中CRH浓度明显降低（P ＜ 0.01），CRH mRNA表达亦显著降低（P ＜ 0.05或P ＜ 0.01），而单穴位组对各监测指标的作用效果不明显（P ＞ 0.05）。结论针刺能缓解酒精戒断焦虑症大鼠的焦虑，其抗焦虑的作用可能与调节大鼠海马中CRH的表达有关。

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	邢宇双1
	2 郑科文2 梁启超2 吴宜艳2 赵玉佳2

关键词 ：针刺, 焦虑, 酒精戒断, 促肾上腺皮质激素释放激素

Abstract：Objective To observe and explore the effect and mechanism of acupuncture for the treatment of alcohol withdrawal anxiety in rats. Methods Fifty-four SPF healthy male SD rats were selected and randomly divided into blank group, model (alcohol withdrawal anxiety model) group, sānyīnjiāo group, zúsānlǐ group, electro-acupuncture two-point (sānyīnjiāo + zúsānlǐ) group, acupuncture two-point (sānyīnjiāo + zúsānlǐ) group, with 9 rats in each group. Except the blank group, all the other groups were given continuous intraperitoneal injection of alcohol for 28 days to prepare the alcohol addiction model, withdrawal of alcohol for 3 days to prepare the alcohol withdrawal anxiety model, and they were respectively given acupuncture at corresponding points during withdrawal of alcohol for 3 days. The elevated plus-maze (EPM) test was used to measure the anxiety behavior. The enzyme-linked immunosorbent assay (ELISA) was used to measure the concentration of corticotropin releasing hormone (CRH) in the hippocampus. The real-time fluorescence quantitative PCR was used to detect the expression of CRH mRNA in hippocampus. Results Compared with the blank group, the percentage of number and residence time of the model group staying in the open arms were significantly reduced (P ＜ 0.05), the concentration of CRH in the hippocampus was significantly increased (P ＜ 0.01), the expression of CRH mRNA was significantly increased (P ＜ 0.01); compared with the model group, the percentage of number of entering the open arm and residence time staying in the open arm during EPM test of the electro-acupuncture two-point group were significantly increased (P ＜ 0.05 or P ＜ 0.01), the percentage of number entering the open arm during EPM test of the acupuncture two-point group was significantly increased (P ＜ 0.05), CRH concentrations in hippocampus of the electro-acupuncture two-point group and the acupuncture two-point group were significantly decreased (P ＜ 0.01), and CRH mRNA expression in hippocampus was also significantly decreased (P ＜ 0.05 or P ＜ 0.01). However, the effect of single acupoint group on each monitoring index was not significant (P ＞ 0.05). Conclusion Acupuncture can relieve the anxiety of rats with alcohol withdrawal anxiety, and its anti-anxiety effect may be related to the regulation of CRH expression in the hippocampus of rats.

Key words： Acupuncture Anxiety Alcohol withdrawal Corticotropin releasing hormone

基金资助:黑龙江省自然科学基金面上项目（H201495）。

通讯作者: 赵玉佳（1970-），男，硕士，副教授，主要从事药物研究。吴宜艳（1963-），女，硕士，教授，主要从事中医药研究。

作者简介: 邢宇双（1993-），女，硕士；研究方向：中医药。

引用本文:

邢宇双1,2 郑科文2 梁启超2 吴宜艳2 赵玉佳2. 针刺对大鼠酒精戒断焦虑症的治疗效果及与CRH的关系[J]. 中国医药导报, 2019, 16(26): 12-16.
XING Yushuang1,2 ZHENG Kewen2 LIANG Qichao2 WU Yiyan2 ZHAO Yujia2. Effect of acupuncture for the treatment of alcohol withdrawal anxiety in rats and its relationship with CRH. 中国医药导报, 2019, 16(26): 12-16.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I26/12

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