1.Department of Clinical Laboratory, Maternal and Child Health Hospital of Yongchuan District, Chongqing 402160, China;
2.Department of Clinical Laboratory, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China;
3.Department of Clinical Laboratory, Coal Occupational Disease Hospital of Chongqing, Chongqing 402160, China
Abstract：Objective To develop a electrochemical sensor based on multiwalled carbon nanotube-graphene for detection of the rubella virus IgM antibody and to evaluate its analytical performance. Methods Oxidized graphene (GS), chitosan (CHI), multi-wall carbon nano-tubes (MWCNT), 1-butyl-3-methylimidazolium hexafluorophosphate (six-[BMIM][PF6]-NH2), gold nanoparticles and thionine biological nanomaterial were used as materials to modify screen printing electrode by layer-by-layer self-assembly and then rubella antigen was immobilized on the assembled electrode surface, afterwards coated by horseradish peroxidase and eventually formed the electrochemical sensor used in this study. The layer-by-layer assembled electrode was scanned by cyclic voltammetry to detect its electrochemical performance. The optimum experimental conditions of the sensor were obtained by optimization experiments. The analytical performance of the sensor was evaluated under the optimum experimental conditions. Results After the bare electrode was assembled with above-mentioned materials, the peak current and conductivity of the electrode gradually increased, and the response current gradually decreased after being coated by antigen and horseradish peroxidase. The optimum pH value and H2O2 concentration were 7.0 and 8.0 mmol/L respectively. The optimum incubation temperature and time were 35.0℃ and 30 minutes respectively. The repeatability precision and inter-day precision of the detection of rubella virus IgM antibody were 3.4% and 4.9% respectively, the linear range was 0.1-300 pg/mL, the detection limit was 40 fg/mL, the recovery rate was 97.5%. And at the same time, the sensor had a good anti-interference performance. Conclusion Under the optimum experimental conditions, the assembled electrochemical sensor for detection of rubella virus has the advantages of higher sensitivity, better precision and accuracy, the rapid and simple operation and also quantifiability compared with the existing methods.
2017, Vol. 14 
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