依达拉奉对海水淹溺性大鼠急性肺损伤治疗作用的影响

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摘要目的观察依达拉奉对海水淹溺性大鼠急性肺损伤的治疗作用，并探讨其作用机制。方法根据随机数字表法将40只健康雄性SD大鼠分为空白对照组（C组）、依达拉奉组（E组）、海水淹溺组（S组）和治疗组（SE组），每组10只。气管内注入人工海水建立动物模型：C组暴露气管后尾静脉注射2 mL/kg生理盐水；E组暴露气管后尾静脉注射2 mL/kg依达拉奉注射液；S组暴露气管后于气管内注入4 mL/kg人工海水+尾静脉注入2 mL/kg生理盐水；SE组暴露气管后于气管内注入4 mL/kg人工海水+尾静脉注入2 mL/kg依达拉奉注射液。造模6 h后处死大鼠，检测各组大鼠肺组织湿/干比、支气管肺泡灌洗液蛋白含量、血清白细胞介素（IL）-6、IL-10、肿瘤坏死因子-α（TNF-α）含量和肺组织病理改变；RT-PCR及Western blot检测水通道蛋白（AQP）1和AQP5 mRNA及蛋白表达量。结果 S组大鼠肺组织湿干比明显高于C组（P ＜ 0.05）；SE组大鼠肺组织湿干比低于S组（P ＜ 0.05）。S组大鼠支气管肺泡灌洗液蛋白含量明显高于C组（P ＜ 0.05）；SE组大鼠支气管肺泡灌洗液蛋白含量低于S组（P ＜ 0.05）。S组和SE组大鼠血清IL-6、IL-10、TNF-α含量均明显高于C组（P ＜ 0.05）；SE组大鼠血清IL-6、TNF-α含量明显低于S组，血清IL-10含量明显高于S组（P ＜ 0.05）。S组大鼠肺组织AQP1和AQP5 mRNA和蛋白表达量高于C组（P < 0.05）；SE组大鼠肺组织AQP1和AQP5 mRNA和蛋白表达量低于S组（P < 0.05）。结论依达拉奉可通过调节AQP1和AQP5的表达减轻海水淹溺造成的急性肺损伤。

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	陈卓1 山淇1 李宏2

关键词 ：依达拉奉, 淹溺, 急性肺损伤, 水通道蛋白

Abstract：Objective To observe the therapeutic effect of Edaravone on acute lung injury in seawater drowning rats and explore its mechanism. Methods Forty healthy male SD rats were divided into control group (group C), Edaravone group (group E), seawater drowning group (group S) and treatment group (group SE) according to random number table method, with 10 rats in each group. Artificial seawater was injected into trachea to establish animal models: group C was injected with 2 mL/kg saline via tail vein after tracheal exposure; group E was injected with 2 mL/kg Edaravone injection via tail vein after tracheal exposure; group S was injected with 4 mL/kg artificial seawater after tracheal exposure + 2 mL/kg saline via tail vein; group SE was injected with 4 mL/kg artificial saline via trachea after tracheal exposure + 2 mL/kg Edaravone injection via tail vein. Six hours after the establishment of the model, rats were killed. The wet/dry weight ratio, protein concentration in bronchoalveolar lavage fluid, interleukin (IL)-6, IL-10, serum tumor necrosis factor-α (TNF-α) content and pathological changes of lung tissue were detected. The mRNA and protein expression of aquaporin (AQP)-1 and AQP5 were detected by RT-PCR and Western blot. Results The wet-dry ratio of lung tissue in group S was significantly higher than that in group C (P < 0.05), and that in group SE was lower than that in group S (P < 0.05). The protein content of bronchoalveolar lavage fluid in group S was significantly higher than that in group C (P < 0.05); the protein content of bronchoalveolar lavage fluid in group SE was lower than that in group S (P < 0.05). The serum levels of IL-6, IL-10 and TNF-α in group S and SE were significantly higher than those in group C (P < 0.05); the serum levels of IL-6 and TNF-α in group SE were significantly lower than those in group S, and the serum level of IL-10 was significantly higher than that in group S (P < 0.05). The expression of AQP1 and AQP5 in lung tissue of rats in group S was higher than that in group C (P < 0.05); the expression of AQP1 and AQP5 in lung tissue of rats in group SE was lower than that in group S (P < 0.05). Conclusion Edaravone can alleviate the acute lung injury caused by seawater drowning by regulating the expression of AQP1 and AQP5.

Key words： Edaravone Drowning Acute lung injury Aquaporin

基金资助:天津市科技计划项目（15ZXLCSY00040）。

通讯作者: 李宏（1968-），男，硕士，主任医师；研究方向：脏器保护。

作者简介: 陈卓（1985-），男，武警后勤学院2016级外科学专业在读硕士研究生；研究方向：肺损伤的治疗。

引用本文:

陈卓1 山淇1 李宏2. 依达拉奉对海水淹溺性大鼠急性肺损伤治疗作用的影响[J]. 中国医药导报, 2019, 16(23): 16-20.
CHEN Zhuo1 SHAN Qi1 LI Hong2. Effect of Edaravone on acute lung injury in seawater drowning rats. 中国医药导报, 2019, 16(23): 16-20.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I23/16

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