miRNA-494与慢性心力衰竭心肌纤维化相关性的生物信息学分析

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摘要目的利用生物信息学分析方法，探讨miRNA-494与慢性心力衰竭患者心肌纤维化机制的联系。方法利用GEO数据库筛选出GSE104150数据集，分析慢性心力衰竭患者及健康对照者差异表达miRNA，对其中miR-494预测靶基因及靶基因集分别进行基因本体（GO）富集分析及信号通路富集分析。结果与对照者比较，miR-494在慢性心力衰竭患者样本中呈差异性表达。在细胞质、细胞核等细胞组成，蛋白质结合等分子功能，细胞增殖与迁移、磷脂酰肌醇介导的信号传导等生物功能，以及多条心肌纤维化、慢性心力衰竭相关信号通路如转化生长因子-β、PI3K/Akt、MAPK等信号通路中富集。结论推测miR-494在慢性心力衰竭患者中差异性表达，并与心肌纤维化发生发展有一定相关性，有待进一步研究证实。

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	梁珊1 何亚州1 李丽娟1 黄彩依1 王庆高2

关键词 ： miRNA, 心肌纤维化, 慢性心力衰竭, 生物信息学

Abstract：Objective To explore the mechanism of myocardial fibrosis in chronic heart failure patients by using bioinformatics analysis. Methods The GSE104150 dataset through GEO database was filtered out, the differentially expressed miRNAs between heart failure and healthy control was analyzed. The target genes of miR-494 were predicted, which should be performed the gene ontology (GO) enrichment analysis and signal pathway enrichment analysis. Results Compared with the healthy control, miRNA-494 was differentially expressed in the chronic heart failure patients. The GO analysis showed the target genes of miR-494 were enriched in cell components such as cytoplasm and nucleus, molecular functions as protein binding and biological functions as cell proliferation and migration, phosphatidylinositol-mediated signaling. The KEGG biological pathway was mainly enriched in TGFβ, PI3K/Akt, and MAPK signaling pathways. Conclusion It is speculated that miRNA-494 is differentially expressed in patients with chronic heart failure, and it has a certain correlation with the occurrence and development of myocardial fibrosis, further research is needed to confirm.

Key words： MiRNA Myocardial fibrosis Chronic heart failure Bioinformatics analysis

基金资助:国家自然科学基金资助项目（81160437）；
广西中医药大学第一附属医院科研项目（2014年广西高校重点学科结余经费）（2017XK03）。

通讯作者: 王庆高（1975-），男，博士，主任医师，主要从事临床心血管疾病治疗及研究。

作者简介: 梁珊（1992-），女，广西中医药大学2016级中医内科学专业在读硕士研究生；研究方向：中西医结合防治心血管疾病。

引用本文:

梁珊1 何亚州1 李丽娟1 黄彩依1 王庆高2. miRNA-494与慢性心力衰竭心肌纤维化相关性的生物信息学分析[J]. 中国医药导报, 2019, 16(23): 7-11.
LIANG Shan1 HE Yazhou1 LI Lijuan1 HUANG Caiyi1 WANG Qinggao2. Bioinformatics analysis of the relationship between miRNA-494 and myocardial fibrosis in chronic heart failure. 中国医药导报, 2019, 16(23): 7-11.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I23/7

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