贯叶金丝桃提取物对小鼠行为学的影响

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摘要目的探讨贯叶金丝桃提取物（ESJWT）对小鼠行为学的影响。方法选取30只昆明小鼠，根据随机数字表法分为三组，每组10只，对照组给予等量纯化水，ESJWT低、高剂量组给予等量的供试品，连续给药14 d，测定小鼠自转棒掉落情况。选取50只昆明小鼠，根据随机数字表法分为对照组和ESJWT低、高剂量组，每组8只。对照组给予等量纯化水，ESJWT低、高剂量组给予等量的供试品，连续给药14 d，测试并记录小鼠5 min的自主活动量。结果三组小鼠给药后的转棒实验潜伏期比较，差异无统计学意义（P ＞ 0.05）。ESJWT低、高剂量组小鼠活动总路程较对照组明显缩短，差异有高度统计学意义（P ＜ 0.01）。ESJWT低、高剂量组小鼠四周活动路程较对照组短，ESJWT高剂量组小鼠四周活动进入次数较对照组少，差异有统计学意义（P ＜ 0.05）。ESJWT高剂量组小鼠中心活动持续时间较ESJWT低剂量组、对照组短，ESJWT高剂量组小鼠中心活动进入次数较对照组少，ESJWT低、高剂量组小鼠中心活动潜伏期较对照组短，差异有统计学意义（P ＜ 0.05或P < 0.01）。结论 ESJWT可使小鼠自发活动量减少，对昆明小鼠的转棒实验无明显影响。

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	傅松年1 阿不都克热木·阿不都热合曼2 罗福祥3 祖力喀尔·买买提3 马丽3 李治建3

关键词 ：贯叶金丝桃提取物, 小鼠, 中枢神经系统, 行为学

Abstract：Objective To investigate the effects of Hypericum perforatum extract (ESJWT) on of behavior in mice. Methods Thirty Kunming mice were randomly divided into three groups according to the random number table method, with 10 mice in each group. The control group was given the same amount of purified water, and the ESJWT low and high dose groups were given the same amount of samples for 14 days. The drop of the rotating rod in mice was determined. 50 Kunming mice were randomly divided into control group and ESJWT low-dose and high-dose groups with 8 mice in each group. The control group was given the same amount of purified water, and the ESJWT low-dose and high-dose groups were given the same amount of samples for 14 days. The voluntary activity of mice for 5 minutes was measured and recorded. Results There was no significant difference in the latency of rod rotation test among the three groups (P > 0.05). Total activity distance of ESJWT mice in low and high dose groups was significantly shorter than that in control group, the difference was highly statistically significant (P < 0.01). All around activity distance of mice in low and high dose ESJWT group was shorter than that in control group, the number of all around activity entry in ESJWT high-dose group was less than that in control group, the difference was statistically significant (P < 0.05). The duration of central activity in ESJWT high dose group was shorter than that in ESJWT low-dose group and control group, the number of central activity entry in ESJWT high-dose group was less than that in control group, the latency of central activity in ESJWT low and high dose group was shorter than that in control group, the difference was statistically significant (P < 0.05 or P < 0.01). Conclusion ESJWT can reduce the spontaneous activity of mice, and has no significant effect on the rod test of Kunming mice.

Key words： Hypericum perforatum extract Mice Central nervous system Behavior

基金资助:国家重点研发计划中医药现代化研究专项课题（2018YFC1708006-3）；
国家“重大新药创制”科技重大专项课题（2017ZX09301060005）。

通讯作者: 马丽（1983-），女，硕士；研究方向：药理毒理学。李治建（1982-），男，硕士，副研究员；研究方向：药理毒理学。

作者简介: 傅松年（1970-），男，硕士，副教授，副主任医师；研究方向：精神药理学，社区精神卫生。

引用本文:

傅松年1 阿不都克热木·阿不都热合曼2 罗福祥3 祖力喀尔·买买提3 马丽3 李治建3. 贯叶金丝桃提取物对小鼠行为学的影响[J]. 中国医药导报, 2019, 16(21): 17-20.
FU Songnian1 Abudoukeremu·Abudoureheman2 LUO Fuxiang3 Zulikaer·Maimaiti3 MA Li3 LI Zhijian3. Effects of Hypericum perforatum extract on mice behavior. 中国医药导报, 2019, 16(21): 17-20.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I21/17

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