Effect of Shugan Jieyu Capsules on brain-derived neurotrophic factor in model rats with depression
WANG Hanyan GUO Dongmei TANG Zhen
Teaching and Research Section of Biochemistry, School of Basic Medical Sciences, North Sichuan Medical College, Sichuan Province, Nanchong 637000, China
Abstract：Objective To investigate the effect of Shugan Jieyu (SGJY) Capsules on brain-derived neurotrophic factor (BDNF) in model rats with depression. Methods Thirty SD rats were randomly divided into five groups (n = 6) by random number table method: control group, chronic unpredictable mild stress (CUMS) group, Fluoxetine (FLU) group (FLU 2 mg/kg), SGJY group (SGJY Suspension Liquid 150 mg/kg) and SGJY+U0126 group (SGJY Suspension Liquid 150 mg/kg and 0.01% U0126 5 mL/kg). The CUMS was performed to induce depressive-like animal model. Each group was given corresponding drugs for 21 d. The control group and CUMS group were given normal saline 5 mL/kg by gavage. All animals were taken behavior tests. The expression of BDNF mRNA was detected by real-time PCR, the expression of pERK1/2 protein was determined by Western blot. Results Compared with control group, the times of passing through grille, the upright times and sugar preference in the rest groups were all reduced before administration (P ＜ 0.01). After administration, compared with CUMS group, the times of passing through grille, the upright times and sugar preference in FLU group and SGJY group were increased significantly (P ＜ 0.01 or P ＜ 0.05), the expression of BDNF mRNA, pERK1/2 was improved significantly (P ＜ 0.01); compared with SGJY group, the times of passing through grille, the upright times and sugar preference in SGJY+U0126 group were decreased signfiicantly (P ＜ 0.01 or P ＜ 0.05), the expression of BDNF mRNA, pERK1/2 was reduced significantly (P ＜ 0.01 or P ＜ 0.05). Conclusion SGJY Capsules may play a role in improving the depressive symptoms by regulating the ERK1/2 pathway to increase the expression of BDNF.
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2019, Vol. 16 
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