赤芍乙醇提取物和水提取物抗病毒研究

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摘要目的研究赤芍乙醇提取物和水提取物的抗病毒活性。方法结合细胞病变效应法（CPE）及CCK-8试剂盒，以治疗指数（TI）为指标，研究赤芍乙醇和水提取物对呼吸道合胞病毒（RSV）、单纯疱疹病毒（HSV-1）、柯萨奇病毒（COX-B5）、手足口病毒（EV71）的预防、直接杀灭和穿入后抑制作用。结果赤芍乙醇和水提取物对RSV、HSV-1、COX-B5、EV71没有预防和穿入后的阻断作用，对EV71没有直接杀灭作用，对RSV、HSV-1、COX-B5有直接杀灭作用，水提取物对RSV和COX-B5直接杀灭作用效果较好，TI值分别为15.034和13.076；75%乙醇提取物对HSV-1的直接杀灭作用较好，TI值为10.944；阳性对照药对四种病毒均有作用，其中利巴韦林对RSV直接杀灭、预防和穿入后阻断作用的TI值分别为19.760、16.760、18.028，对COX-B5的TI值分别为17.482、15.516、16.568，对EV71的TI值分别43.158、24.296、22.162；阿昔洛韦对HSV-1直接杀灭、预防和穿入后阻断作用的TI值分别为23.432、10.922、23.417。结论赤芍乙醇和水提取物对RSV、HSV-1、COX-B5具有直接杀灭作用，对EV71无直接杀灭作用，对四种病毒无预防和穿入后阻断作用，阳性对照药对四种病毒具有直接杀灭、预防和穿入后阻断作用。

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	刘相文侯林张成华孙纯勇刘金安田景振

关键词 ：赤芍, 抗病毒, 呼吸道合胞病毒, 单纯疱疹病毒, 柯萨奇病毒

Abstract：Objective To investigate the antiviral activity of ethanol extract and water extract of Paeoniae Radix Rubra. Methods Combining with cytopathic effect (CPE) method and CCK-8, taking therapeutic index (TI) as index, the prevention, direct killing and penetration inhibition effects of ethanol extract and water extract of Paeoniae Radix Rubra for respiratory syncytial virus (RSV), herpes simplex virus (HSV-1), Coxsackie virus (COX-B5), hand, foot and mouth virus (EV71) were researched. Results The ethanol extract and water extract of Paeoniae Radix Rubra had no prevention and penetration inhibition effects for RSV, HSV-1, COX-B5, EV71, which had no direct killing effects for EV71 and had direct killing effects for RSV, HSV-1, COX-B5, among which, the water extract had better direct killing effects for RSV and COX-B5, the TI value was 15.034 and 13.076 respectively. 75% ethanol extract of Paeoniae Radix Rubra had good direct killing effect for HSV-1, the TI value was 10.944, all positive control drugs had effects for the four kinds of virus, among which, the TI values of Ribavirin for the direct killing, prevention and penetration inhibition effects of RSV were 19.760, 16.760, 18.028 respectively, which were 17.482, 15.516, 16.568 for COX-B5, 43.158, 24.296, 22.162 for EV71. The TI values of Acyclovir for the direct killing, prevention and penetration inhibition effects of HSV-1 were 23.432, 10.922, 23.417 respectively. Conclusion The ethanol extract and water extract of Paeoniae Radix Rubra has direct killing effects for RSV, HSV-1, COX-B5, has no direct killing effects for EV71, has no prevention and penetration inhibition effects for four kinds of virus, while the positive control drugs have direct killing, prevention and penetration inhibition effects for the four kinds of virus.

Key words： Paeoniae Radix Rubra Antiviral Respiratory syncytial virus Herpes simplex virus Coxsackie virus

基金资助:国家科技重大新药创制专项（2014ZX09509001）；
山东省高校中医药抗病毒协同创新中心课题（XTCX2014C01-01）。

通讯作者: 田景振（1957-），男，博士，教授，山东中医药大学药学院院长，从事中药炮制原理与新药研发。

作者简介: 刘相文（1992-），男，山东中医药大学2015级中药学在读硕士研究生，从事中药炮制原理与新药研发。

引用本文:

刘相文侯林张成华孙纯勇刘金安田景振. 赤芍乙醇提取物和水提取物抗病毒研究[J]. 中国医药导报, 2017, 14(16): 4-7.
LIU Xiangwen HOU Lin ZHANG Chenghua SUN Chunyong LIU Jin′an TIAN Jingzhen. Study on the antiviral activity of ethanol extract and water extract of Paeoniae Radix Rubra. 中国医药导报, 2017, 14(16): 4-7.

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