Abstract：Objective To prepare a nano-scale ultrasound molecular probe carrying hypocrellin (H) and targeting folic acid (FA) molecule, and to investigate its basic characteristics and in vitro targeting ability. Methods H and polylactic acid-glycolic acid (PLGA) were dissolved in organic solvents in a certain proportion. Nano-scale ultrasound contrast agent (PLGA/H) containing H was prepared by double emulsification method. The basic characteristics, entrapment efficiency and drug loading of PLGA/H were observed and measured, and the optimal dosage of H was optimized. After the FA molecule was attached to the surface of PLGA by adding folate-conjugated phospholipid DSPE-PEG (2000) Floate, nanoparticles with both H and FA molecular targeting function (PLGA-FA) were prepared by double emulsion method. PLGA/H and PLGA-FA/H were respectively applied to human nasopharyngeal carcinoma (NPC) cell lines CNE2 (high expression of FA receptor) and CNE2 (low expression of FA receptor) in vitro to observe and compare their targeting. Results The nanoparticle PLGA/H carrying H was successfully prepared. The average particle size of the nanoparticles was (220.50±91.37) nm, the shape was round, the size was uniform and the dispersion was good. PLGA-FA/H nanoparticles can be targeted to CNE2 cells (high expression of FA receptor) of human NPC cell line, while CNE2 cells (low expression of FA receptor) with NPC cell line have no targeted binding. Conclusion PLGA-FA/H, a kind of contrast-enhanced ultrasound nanoparticle with H and FA molecular targeting function, has been successfully prepared, and it can target CNE2 cells (high expression of FA receptor) of human NPC cell line.
王萍 岳文胜 唐雪梅 罗玉群 李祖坤. 载竹红菌素纳米靶向探针的制备及其体外对NPC细胞寻靶能力的实验研究[J]. 中国医药导报, 2019, 16(19): 16-19,33.
WANG Ping YUE Wensheng TANG Xuenei LUO Yuqun LI Zukun. Experiment study of preparation of homomycin nanoparticle targeting probe and its ability to target NPC cells in vitro. 中国医药导报, 2019, 16(19): 16-19,33.
[1] Carioli G，Negri E. Global trends in nasopharyngeal cancer mortality since 1970 and predictions for 2020：Focus on low-risk areas [J]. Int J Cancer，2017，140：2256-2264.
[2] Song J，Wang Y，Fan X，et al. Trans-vaccenic acid inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells via a mitochondrial-mediated apoptosis pathway [J]. Lipids Health Dis，2019，18（1）：46.
[3] Zhang L，Huang Y，Hong S，et al. Gemcitabine plus cisplatin versus fluorouracil plus cisplatin in recurrent or metastatic nasopharyngeal carcinoma：a multicentre，randomised，open-label，phase 3 trial [J]. Lancet，2016，388：1883-1892.
[4] Alibolandi M，Abnous K，Sadeghi F，et al. Folate receptor-targeted multimodal polymersomers for delivery of quantum dots and doxorubicin to breast adenocarcinoma：In vitro and in vivo evaluation [J]. Int J Pharm，2016，500（1/2）：162-178.
[5] Liu R，Hu C，Yang Y，et al. Theranostic nanoparticles with tumor-specific enzyme-triggered size reduction and drug release to perform photothermal therapy for breast cancer treatment [J]. Acta Pharm Sin，2019，9（2）：410-420.
[6] Konopka CJ，Wozniak M，Hedhli J，et al. Multimodal imaging of the receptor for advanced glycation end-products with molecularly targeted nanoparticles [J].Theranostics，2018，8（18）：5012-5024.
[7] Li Y，Li D，Jian K，et al. A Synergistically Enhanced T1 and T2-Weighted Magnetic Resonance/Near-Infrared Contrast Agent of Gd-Doping Fe3O4@Fluorescence PEGylated Nanoparticles for Multimodality Imaging of Hepatocarcinoma [J]. J Biomed Nanotechnol，2019，15（1）：85-99.
[8] van Onzen AHAM，Rossin R，Schenning APHJ，et al. Tetrazine- trans-Cyclooctene Chemistry Applied to Fabricate Self-Assembled Fluorescent and Radioactive Nanoparticles for in Vivo Dual Mode Imaging [J]. Bioconjug Chem，2019，30（3）：547-551.
[9] Shi H，Sun Y，Yan R，et al. Magnetic Semiconductor Gd-Doping CuS Nanoparticles as Activatable Nanoprobes for Bimodal Imaging and Targeted Photothermal Therapy of Gastric Tumors [J]. Nano Lett，2019，19（2）：937-947.
[10] Ping Wang，Chuanshan Xu，Jing Xu，et al. Hypocrellin B enhances ultrasound-induced cell death of nasopharyngeal carcinoma cells [J]. Ultrasound Med Biol，2010，36（2）：336-342.
[11] 徐芬芬，王志刚，李攀，等.载10-羟基喜树碱液态氟碳纳米粒的声致相变条件及释药性能[J].中国医学影像技术，2015，31（2）：196-199.
[12] You Y，Wang Z，Ran H，et al. Nanoparticle-enhanced synergistic HIFU ablation and transarterial chemoembolization for effcient cancer therapy [J]. Nanoscale，2016，8（7）：4324-4339.
[13] Han FY，Thurecht KJ，Whittaker AK，et al. Biodegradable PLGA-based microparticles for producing sustained-release drug formulations and strategies for improving drug loading [J]. Front Pharmacol，2016，7：185.
[14] Baishya R，Nayak DK，Kumar D，et al. Ursolic acid loaded PLGA nanoparticles：in vitro and in vivo evaluation to explore tumor targeting ability on B16F10 melanoma cell lines [J]. Pharm Res，2016，33（11）：2691-2703.
[15] Aldayel AM，Naguib YW，O'Mary HL，et al. Acid-sensitive sheddable PEGylated PLGA nanoparticles increase the delivery of TNF-α siRNA in chronic inflammation sites [J]. Mol Ther Nucleic Acids，2016，5（7）：e340.
[16] 杨恒丽，蔡文斌，张莉，等.纳米级分子靶向超声造影剂的制备及其体外肿瘤靶向性实验研究[J].中国超声医学杂志，2016，32（4）：367-70.
[17] Zhong L，Zou H，Huang Y，et al. Magnetic Endoglin Aptamer Nanoprobe for Targeted Diagnosis of Solid Tumor [J]. J Biomed Nanotechnol，2019，15（2）：352-362.
[18] Davis RM，Campbell JL，Burkitt S，et al. A Raman Imaging Approach Using CD47 Antibody-Labeled SERS Nanoparticles for Identifying Breast Cancer and Its Potential to Guide Surgical Resection [J]. Nanomaterials（Basel），2018，8（11）. pii：E953.
[19] Liu T，Qiao Z，Wang J，et al. Molecular imprinted S-nitrosothiols nanoparticles for nitric oxide control release as cancer target chemotherapy [J]. Colloids Surf B Biointerfaces，2019，173：356-365.
[20] Allahyari M，Mohit E. Peptide/protein vaccine delivery system based on PLGA particles [J]. Hum Vaccin Immunother，2016，12（3）：806-828.
[21] 吴汤娜，王东林，景香香.液态氟碳纳米粒在超声分子靶向成像及治疗中的研究进展[J].临床超声医学杂志，2016，18（11）：761-63.
2019, Vol. 16 
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