Effect of Fibrauretin on the protein expression and activation of NF-κB in lung tissues of mice with acute lung injury
LI Li1 WANG Yingxia2 YANG Guimei3 YIN Jianbin3 ZHANG Xuan3
1.Biotherapy Center, the 3rd Affiliated Hospital of Kunming Medical University Tumor Hospital of Yunnan Province, Yunnan Province, Kunming 650106, China; 2.Department of Pathology, the First Affiliated Hospital of Kunming Medical University, Yunnan Province, Kunming 650032, China;
3.School of Pharmaceutical Sciences & Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Yunnan Province, Kunming 650500, China
Abstract：Objective To study the effect of Fibrauretin on the protein expression and activation of nuclear factor kappa B (NF-κB) in lung tissues of mice with acute lung injury (ALI). Methods Sixty male SPF Kunming mice were randomly divided into 6 groups by drawing lots (10 mice in each group): ALI group [(intratracheal instillation of 5 mg/kg lipopolysaccharide (LPS)], normal control group (sham operation), positive control group (3.3 mg/kg Hydrocortisone Injection + LPS), low, medium and high doses of Fibrauretin groups (2, 10 mg/kg and 50 mg/kg Fibrauretin Injection + LPS). The positive drug and Fibrauretin were given by intraperitoneal injection once a day for 3 consecutive days before establishment of ALI model. Immunohistochemistry was used to detect the expression and localization of NF-κB p65 protein in lung tissues. Western blot was used to detect the expression of phosphorylated NF-κB p65 protein in lung tissues. Results Compared with ALI group, the percentage of positive cells with the entry of NF-κB p65 protein into the nucleus in lung tissues of mice in medium and high doses of Fibrauretin groups was decreased significantly, the difference was statistically significant (P < 0.05). Compared with ALI group, the protein expression of phosphorylated NF-κB p65 in lung tissues of mice in low, medium and high doses of Fibrauretin group was down-regulated significantly, the differences were statistically significant (P < 0.05 or P < 0.01). Conclusion Fibrauretin has preventive effect on LPS-induced acute lung injury in mice, and the effect is related to the inhibition of the protein expression and activation of NF-κB.
李莉1 王应霞2 杨桂梅3 尹健彬3 张旋3. 黄藤素对急性肺损伤小鼠肺组织NF-κB蛋白表达和激活的影响[J]. 中国医药导报, 2019, 16(19): 7-10.
LI Li1 WANG Yingxia2 YANG Guimei3 YIN Jianbin3 ZHANG Xuan3. Effect of Fibrauretin on the protein expression and activation of NF-κB in lung tissues of mice with acute lung injury. 中国医药导报, 2019, 16(19): 7-10.
2019, Vol. 16 
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