Study of simulated microgravity affecting antitumor response of mesenchymal stem cells against A549 transplantation tumor
LI Jing1 CHEN Jun2 LI Xiuyu1 NIU Lufang1
1.Department of Traditional Chinese Medicine, PLA Navy General Hospital, Beijing 100048, China; 2.Department of Acupuncture and Massage, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China
Abstract：[Abstract] Objective To estimate the whole cell antigens (WCAs) of mesenchymal stem cells (MSCs) cultured in different gravity can generate different immune response against cancer in mice which induced by lung cancer cell line A549. Methods The MSCs was isolated and cultured adherent cells from marrow and a 2-dimensional clinostat (2D clinostat) was used to keep cells in continuous 2D rotation at a speed of 30 r/min around the horizontal axis, to mimc the microgravity (MMG), and the normal gravity (NG) was used as control. And then MSCs was to irradiate to X-ray (15 Gy) to get the WCAs. The experiment group (MMG group) was immunized with WACs (once/3 d, 2 weeks) and then the mice were challenged with A549 cells; the control mice immunized subcutaneously with PBS control (PBS group), MSCs-NG control (NG group) and A549 control (A549 group), mice were monitored for their tumor growth by measure the diameter; then the weight and volume was plotted; the expression of PCNA was monitored by immunohistochemisty. Results A well-established lung cancer (A549) model was established, MMG could improve the immune responses against A549 lung carcinoma (P < 0.05). The A549 group had strongest inhibit effect, while the PBS group had the biggst tumor, the tumor of MMG group and NG group were found beginning to grow until 6 d; and the size in MMG group was smaller than NG group (P < 0.05); the weight of tumor were measured on 12 d, it showed that the weight of NG group was (458.7±21.6) g, while in MMG was (315.6±18.5) g; the resist effet in MMG group was bigger than NG group (P < 0.05). HRP stain showed MMG could down-regulated the PCNA expression (P < 0.05). Conclusion Immunization of with MSCs getting WCAs can suppress tumor growth, MMG can enhance this suppressing effect, it can be observed PCNA expression down, the further immune mechanism need more exploring.
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