Abstract:Objective To investigate the synergistic effects of salt loading and aldosterone on hypertrophy of primary cardiomyocytes in neonatal rats. Methods Neonatal rat cardiomyocytes were culturedin the experiment, the cell was divided into normal control group, Aldosterone 10-7 mol/L (Ald) group, normal sodium concentration 140 mmol/L (NS) group, high sodium concentration 146 mmol/L (HS) group, 140 mmol/L+Ald 10-7 mol/L (NS+Ald) group, 146 mmol/L+Ald 10-7mol/L (HS+Ald) group. At first Ald was not added into (NS+Ald) group and (HS+Ald) group, after 48 h culture, Ald was added, then all treatment groups were continued to culture for 24 h. The difference of myocardial cross-sectional area was measured by immunofluorescence. The survival rate was detected by CCK-8. The apoptosis rate was detected by flow cytometry. ET-1 and eNOS mRNA expressions were assayed by qRT-PCR. Results The cross-sectional area of myocardial cells in NS+Ald group and HS+Ald group were significantly higher than Ald group, NS group, HS group. Compared with normal control group, the survival rate of cardiomyocytes decreased significantly (P < 0.01) and apoptosis rate increased significantly (P < 0.01) in NS+Ald group and HS+Ald group; except for NS group, ET-1 mRNA expression increased and eNOS mRNA expression decreased in other groups, the effect of HS+Ald group was the most significant (P < 0.01). Conclusion Salt loading and aldosterone are important participation factors which induced cardiomyocyte hypertrophy, they have synergistic effects. Among them, aldosterone-induced cardiomyocyte hypertrophy is the most significant in high salt condition.
由淑萍1 蒋红1 何丽娟2 陶宁2 王忠2 陈清杰3 刘涛2. 盐负荷和醛固酮诱导乳鼠原代心肌细胞肥大的实验研究[J]. 中国医药导报, 2017, 14(28): 13-16.
YOU Shuping1 JIANG Hong1 HE Lijuan2 TAO Ning2 WANG Zhong2 CHEN Qingjie3 LIU Tao2. Experimental research on effect of hypertrophy of primary cardiomyocytes in suckling mouse induced by salt loading and aldosterone. 中国医药导报, 2017, 14(28): 13-16.
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