Abstract：Objective To study the effects of 3-n-butylphthalide (NBP) on memory and Caspase-3 expression in the hippocampal CA1 region of vascular dementia rats, in order to explore protective effects and mechanisms of NBP on rats with vascular dementia (VD). Methods A total of 80 SPF Wistar rats were divided into vascular dementia models group (VD group), vascular dementia models + NBP injection group (NBP treatment group), sham surgery + NBP injection group (NBP control group), sham surgery group (Sham group), according to random group method. Then each group was divided into four subgroups (n = 5): 1, 2, 4, and 8 weeks after surgery. VD models were established by ligating bilateral common carotid artery. Then the rats in the NBP treatment group and NBP control group were intraperitoneally injected with NBP 5 mg/（kg·d） for 7 consecutive days. The rats in the VD group and Sham group were intraperitoneally injected with saline (0.2 mL/d) for 7 consecutive days. Memory ability of 8 weeks subgroup rats were tested by Morris water maze at 4 and 8 weeks after surgery. At 1, 2, 4, and 8 weeks after surgery, the rats in each group were decapitated. The brains were obtained, and then hippocampus were isolated. Caspase-3 expression in the hippocampal CA1 region were determined by immunohistochemistry. Results Compared with VD group, escape latency in the NBP treatment group were significantly shorter (P < 0.05). At 1, 2, 4 and 8 weeks after surgery, Caspase-3 expression in the VD group and NBP treatment group were significantly increased than those in the Sham group (P < 0.05). Compared with VD group, Caspase-3 expression in the NBP treatment group were significantly lower at 4 and 8 weeks after surgery (P < 0.05). In VD group, Caspase-3 expression at 8 weeks after surgery was significantly higher than at 1 weeks (P < 0.05). Conclusion NBP can improve the memory ability of VD rats, which may be connected with the overexpression of caspase-3 in the hippocampus CA1 region of VD rats.
毛西京 朱博驰 于挺敏 姚刚▲. 丁苯酞对血管性痴呆大鼠记忆能力及海马CA1区Caspase-3表达的影响[J]. 中国医药导报, 2017, 14(28): 9-12.
MAO Xijing ZHU Bochi YU Tingmin YAO Gang▲. Effects of 3-n-butylphthalide on memory and Caspase-3 expression in the hippocampal CA1 region of vascular dementia rats . 中国医药导报, 2017, 14(28): 9-12.
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2017, Vol. 14 
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