Abstract:Objective To establish a HPLC method for simultaneous determination of six compounds (uridine, carnine, guanosine, albiflorin, liquiritin, liquiritin apioside) in Gansuibanxia Decoctions, in order to provide a theoretical basis for the quality control of Gansuibanxia Decoction. Methods The Agilent TC-C18 column (4.6 mm×250 mm×5 μm) was used at 30℃ with acetonitrile-water (gradient elution) as the mobile phase; the detective wavelength was 254 nm; the flow rate was 1.0 mL/min; the injection volume was 20 μL. Results Every standards showed a good linear relationship in the linear range, the correlation coefficients were all more than 0.9995. The recoveries were in the range of 98.1%-101.2%. RSDs were all less than 3.3%. Conclusion The method established in this paper is simple and fast with good accuracy and repeatability. It may lay the foundation for the quality control of Gansuibanxia Decoctions.
李文静 洪博 刘军 张杰 徐天娇 王玉春. 高效液相色谱法用于甘遂半夏汤中6种成分的含量测定[J]. 中国医药导报, 2017, 14(33): 130-132,142.
LI Wenjing HONG Bo LIU Jun ZHANG Jie XU Tianjiao WANG Yuchun. Determination the contents of six compounds in Gansuibanxia Decoctions by high performance liquid chromatography. 中国医药导报, 2017, 14(33): 130-132,142.