褐藻多糖硫酸酯对MPP+损伤的MN9D细胞氧化应激及凋亡的保护作用

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摘要目的探讨褐藻多糖硫酸酯（FUC）对帕金森病（PD）细胞模型的保护作用及机制。方法采用100 μmol/L MPP+损伤的MN9D细胞制作PD模型，用MTS检测FUC预处理后PD细胞模型的细胞存活率。荧光检测法测定细胞内活性氧（ROS），荧光素酶法检测细胞内Caspase-3的活性变化，Western blot检测凋亡相关蛋白Bax及Bcl-2的变化。结果 FUC 100 μmol/L作用24 h明显增加了MPP+损伤的MN9D细胞存活率，差异有高度统计学意义（P ＜ 0.01）；FUC预保护明显降低了MPP+损伤3 h的MN9D细胞内ROS活性及6 h时Caspase-3的表达，差异有高度统计学意义（P ＜ 0.01）；FUC、司来吉兰预保护后MN9D细胞凋亡相关蛋白Bax表达明显下降，而Bcl-2表达明显增加，差异有高度统计学意义（P ＜ 0.01）。结论褐藻多糖硫酸酯可能通过抗氧化、降低Caspase-3表达、抑制细胞凋亡来保护PD细胞模型。

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	梁志刚1 孙旭文1 刘竹丽1 杨绍婉1 罗鼎真2

关键词 ：褐藻多糖硫酸酯, 帕金森病, MN9D细胞, 氧化应激, 细胞凋亡

Abstract：Objective To explore the protective effect of fucoidan (FUC) on the cell model of Parkinson′s disease (PD) and its mechanism. Methods PD model was established by MN9D cells which were damaged by 100 μmol/L MPP+. The cell viability of PD cell model was detected by MTS after FUC pretreatment. The reactive oxygen species (ROS) was measured by fluorescence detection, the activity of Caspase-3 in cells was measured by luciferase, the expression changes of apoptosis-related proteins Bax and Bcl-2 were measured by Western blot. Results After application of 12 h, FUC 100 μmol/L significantly increased the cell viability of MN9D cells damaged by MPP+, the difference was highly statistically significant (P ＜ 0.01). The FUC pretreatment reduced the activity of intracellular ROS of MN9D cell damaged by MPP+ in 3 h and the expression of Caspase-3 in 6 h, there were highly statistically significant differences (P ＜ 0.01). After FUC and Selegiline pretreatment, the expression of MN9D cell apoptosis-related protein Bax was decreased, and the expression of Bcl-2 was increased, the differences were highly statistically significant (P ＜ 0.01). Conclusion Fucoidan may protect the PD cell models through anti-oxidation, reducing the expression of Caspase-3 and inhibiting cell apoptosis.

Key words： Fucoidan Parkinson′s disease MN9D cell Oxidation stress Cell apoptosis

基金资助:山东省烟台市科技发展计划项目（2012077）。

引用本文:

梁志刚1 孙旭文1 刘竹丽1 杨绍婉1 罗鼎真2. 褐藻多糖硫酸酯对MPP+损伤的MN9D细胞氧化应激及凋亡的保护作用[J]. 中国医药导报, 2019, 16(7): 20-24.
LIANG Zhigang1 SUN Xuwen1 LIU Zhuli1 YANG Shaowan1 LUO Dingzhen2. The protective effect of fucoidan on oxidation stress and apoptosis of MN9D cells damaged by MPP+. 中国医药导报, 2019, 16(7): 20-24.

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