Study on separation and purification process of Gardenia jasminoides extract
CUI Long1,2 HU Shaoyin1,2 LIU Heping1,2 SHANG Qiang1,2 GAO Jin1,2
1.Livzon Pharmaceutical Group Co., Ltd., Guangdong Province, Zhuhai 519090, China;
2.National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Province, Zhuhai 519090, China
Abstract：Objective To optimize the separation and purification process parameters of Gardenia jasminoides extract. Methods The transfer rate and color value of crocus sativus glycosides were used as comprehensive scoring indexes. The optimum separation and purification parameters of Gardenia jasminoides extract were optimized by single factor test and orthogonal design, and verified by industrial production. Results The optimum separation and purification process was as follows, the extract was adsorbed by HPD-100A resin at a concentration of 0.5 mg/mL, and then eluted with 12 BV purified water, 2 BV 35% ethanol at a flow rate of 1 BV/h. The Gardenia jasminoides extract was obtained by eluting with 65% ethanol of 4 BV at the same flow rate. Under the optimal process conditions, the pilot production was verified. The average transfer rate, color value and yield of saffron from the three batches of Gardenia jasminoides extract were 86.25%, 509.82 and 2.11%, respectively, and the RSD values were 1.56%, 0.39% and 2.33%. Conclusion The separation and purification process of Gardenia jasminoides extract has been verified by industrial production and is stable and reliable.
崔龙1,2 胡少银1,2 刘和平1,2 尚强1,2 高进1,2. 栀子提取物分离纯化工艺研究[J]. 中国医药导报, 2019, 16(1): 33-37.
CUI Long1,2 HU Shaoyin1,2 LIU Heping1,2 SHANG Qiang1,2 GAO Jin1,2. Study on separation and purification process of Gardenia jasminoides extract. 中国医药导报, 2019, 16(1): 33-37.
2019, Vol. 16 
京公网安备 11010502046598号