柑橘素预处理对大鼠缺血/再灌注心脏损伤后能量代谢的影响

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摘要目的探讨柑橘素预处理对大鼠缺血/再灌注心脏损伤后能量代谢的影响。方法将50只雄性成年Sprague-Dawlay大鼠按照随机数字表法分为五组（n = 10）：空白对照组、模型组和柑橘素低、中、高剂量组（5、10、20 μmol/L）。各组大鼠心脏分别给予30 min全心缺血，然后给予120 min再灌注；柑橘素低、中、高剂量组大鼠分别在缺血前给予5、10、20 μmol/L柑橘素预处理；灌注结束后测定各组大鼠心肌乳酸脱氢酶含量、心肌梗死面积，测定心肌组织Na+/K+-ATP酶和Ca2+/Mg2+-ATP酶的活力。结果缺血再灌注损伤后，与模型组比较，柑橘素低、中、高剂量组乳酸脱氢酶含量下降（P ＜ 0.05或P ＜ 0.01），心肌梗死面积减小（P ＜ 0.05或P ＜ 0.01），心肌组织Na+/K+-ATP酶、Ca2+/Mg2+-ATP酶活力升高（P ＜ 0.05）。结论柑橘素可能通过增强缺血/再灌注大鼠心肌的能量代谢，发挥心脏保护作用。

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	孟利敏

关键词 ：柑橘素, 缺血/再灌注, 能量代谢, 大鼠

Abstract：Objective To investigate the effect of naringenin pretreatment on the energy metabolism after heart injury induced by ischemia/reperfusion in rats. Methods According to random number table method, 50 adult male Sprague-Dawley (SD) rats were divided into five groups (n = 10): blank control group, modle group, and low, medium, high dose of naringenin group (5, 10, 20 μmol/L). The heart of rat in each group was treated with 30 min global ischemia followed by 120 min reperfusion; the rats of low, medium, high dose of naringenin group were treated with 5, 10, 20 μmol/L of naringenin perfusion before ischemia; after reperfusion, the myocardial content of lactate dehydrogenates and myocardial infarct size in each group were examined, the activities of Na+/K+-ATPase and Ca2+/Mg2+-ATPase in myocardial tissue were detected. Results After ischemia/reperfusion injury, compared with modle group, the contents of lactate dehydrogenates in low, medium, high dose of naringenin group were significantly decreased (P ＜ 0.05 or P ＜ 0.01), the myocardial infarct size was significantly reduced (P ＜ 0.05 or P ＜ 0.01), the activities of Na+/K+-ATPase and Ca2+/Mg2+-ATPase in myocardial tissue were significantly improved (P ＜ 0.05). Conclusion Narigenin palys a cardioprotective role may be through enhancing the myocardial energy metabolism of rats with ischemia/reperfusion.

Key words： Naringenin Ischemia/reperfusion Energy metabolism Rats

基金资助:河北省邯郸市科学技术研究与发展计划项目（1623208065-2）。

作者简介: 孟利敏（1978.8-），女，硕士，副主任医师；研究方向：心血管生理学，心血管疾病及各种瓣膜病的诊治。

引用本文:

孟利敏. 柑橘素预处理对大鼠缺血/再灌注心脏损伤后能量代谢的影响[J]. 中国医药导报, 2018, 15(35): 22-25.
MENG Limin. Effect of naringenin pretreatment on the energy metabolism after heart injury induced by ischemia/reperfusion in rats. 中国医药导报, 2018, 15(35): 22-25.

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