Effects of curcumin on the expression of PTP1B and IRS-2 mRNA in liver tissue of rats with type 2 diabetes mellitus
QIN Peijie1 ZHANG Dongwei2 GAO Sihua2▲ LIU Jianfeng1▲
1.Institute of Chinese Medical History Literature, China Academy of Chinese Medical Sciences, Beijing 100700, China;
2.Diabetes Research Center, Beijing University of Chinese Medicine, Beijing 100029, China
Abstract：Objective To observe the effects of curcumin on expression of protein tyrosine phosphatase 1B (PTP1B) and insulin receptor substrate-2 (IRS-2) mRNA in liver tissue of rats with type 2 diabetes mellitus induced by high fat diet. Methods The SD rats were fed with high fat diet, then the tail vein was injected with Streptozotocin (STZ), so as to make type 2 diabetes mellitus model. The model rats were divided into model group, crucumin group, Pioglitazone group, with 10 rats in each group. After 4 weeks and 8 weeks of administration, the levels of fasting blood-glucose (FBG), fasting insulin (FINS), serum total cholesterol (TC), triacylglycerol (TG), low density lipoprotein cholesterin (LDL-C), high density lipoprotein cholesterol (HDL-C) were detected, and the liver index (LI), insulin sensitivity index (ISI) were calculated. After 8 weeks of administration, the pathological changes were observed by HE staining of liver, the mRNA expression of PTP1B and IRS-2 in liver was detected by real-time PCR. Results Compared with model group, crucumin group could significantly reduce the weight and the serum levels of FBG, FINS, LDL-C of rats with type 2 diabetes mellitus (P ＜ 0.05), increase the insulin sensitivity, improve the fatty degeneration and inflammatory reactions of liver tissue, inhibit the expression of PTP1B mRNA, and up-regulate the expression of IRS-2 mRNA (P ＜ 0.05). Conclusion Curcumin can improve the insulin resistance of model rats with type 2 diabetes mellitus, the mechanism may be related to inhibition of PTP1B mRNA expression and up-regulation of IRS-2 mRNA expression.
秦培洁1 张东伟2 高思华2▲ 刘剑锋1▲. 姜黄素对2型糖尿病模型大鼠肝脏组织PTP1B、IRS-2 mRNA表达的影响[J]. 中国医药导报, 2018, 15(34): 14-17.
QIN Peijie1 ZHANG Dongwei2 GAO Sihua2▲ LIU Jianfeng1▲. Effects of curcumin on the expression of PTP1B and IRS-2 mRNA in liver tissue of rats with type 2 diabetes mellitus. 中国医药导报, 2018, 15(34): 14-17.
2018, Vol. 15 
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