环境雌激素对新生仔鼠睾丸引带中Notch信号通路的影响

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摘要目的探讨己烯雌酚（DES）对孕期仔鼠睾丸引带组织中Notch信号通路的影响。方法将45只8~10周龄健康雌性SD大鼠采用随机数字表法分为对照组、实验1组、实验2组，每组15只。实验1、2组分别给予DES 0.1、25.0 μg/（kg·d），按常规雌雄比例2∶1合笼。每组均以仔鼠出生当天记为生后0 d（PD0），分别于生后1 d（PD1）和生后7 d（PD7）时取雄鼠睾丸引带，采用RT-PCR和Western blot测定Notch1、Hes1、表皮生长因子受体（EGFR）和转化生长因子-α（TGF-α）的表达。结果在PD1和PD7时，实验1组中Notch1、Hes1、EGFR和 TGF-α mRNA表达水平均较对照组和实验2组增高（P < 0.05），而实验2组中只有TGF-α mRNA表达水平较对照组增高（P < 0.05）。在PD1时，实验1组中Notch1、Hes1、TGF-α蛋白表达水平较对照组增高，实验2组中Notch1、EGFR、Hes1蛋白表达水平较对照组增高（P < 0.05）；在PD7时，实验1组中Hes1、EGFR蛋白表达水平较对照组增高，实验2组中Notch1、Hes1、EGFR蛋白表达水平较对照组增高（P < 0.05）。与实验1组比较，实验2组PD1时EGFR蛋白表达水平增高，PD7时Notch1、Hes1、TGF-α蛋白表达水平增高（P < 0.05）。结论环境雌激素可影响仔鼠睾丸引带Notch信号通路的表达，可能是外源性雌激素影响睾丸引带发育和增殖的机制之一。

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	王广欢1 陈凯洪1 钟军1 王富生1 许陈斌1 蒋学武2

关键词 ： Notch信号通路, 环境雌激素, 睾丸引带, 表皮生长因子受体

Abstract：Objective To explore the effect of diethylstilbestrol (DES) on Notch signaling pathway of chorda gubernaculum in offspring rats. Methods Forty-five healthy female SD rats aged 8-10 weeks were divided into the control group, experimental group 1 and 2 according to the random number table, with 15 rats in each group. The experimental group 1 and 2 were given DES 0.1 μg/(kg·d) and DES 25 μg/(kg·d) respectively. According to the conventional ratio of male to female 2∶1 cage. The day of offspring birth of each group was recorded as day 0 after birth (PD0). The chorda gubernaculum was taken at PD1 and PD7 respectively. The expressions of Notch1, Hes1, EGFR and TGF-α were analyzed by RT-PCR and Western blot imprinting. Results At PD1 and PD7, the mRNA expression of Notch1, Hes1, EGFR and TGF-α in the experimental group 1 were higher than those in the control group and the experimental group 2 (P < 0.05), while only TGF-α mRNA in the experimental group 2 was higher than that in the control group (P < 0.05). At PD1, the levels of Notch1, Hes1 and TGF-α protein in the experimental group 1 were higher than those in the control group, and the levels of Hes1 and EGFR at PD7 were increased (P < 0.05). Compared with control group, only the levels of Notch1, EGFR and Hes1 protein were in the experimental group 2 increased at PD1, and the levels of Notch1, Hes1 and EGFR protein were increased at PD7 (P < 0.05). Compared with the experimental group 1, only EGFR protein increased in the experimental group 2 at PD1, while Notch1, Hes1 and TGF-α protein increased at PD7 (P < 0.05). Conclusion Environmental estrogen can affect the expression of Notch signaling in gubernaculum of offspring rats, which may be one of the mechanisms of exogenous estrogen affecting the development and proliferation of gubernaculum.

Key words： Notch signaling pathway Environmental estrogen Gubernaculum Epidermal growth factor receptor

基金资助:广东省医学科研基金项目（A2017272）。

引用本文:

王广欢1 陈凯洪1 钟军1 王富生1 许陈斌1 蒋学武2. 环境雌激素对新生仔鼠睾丸引带中Notch信号通路的影响[J]. 中国医药导报, 2018, 15(33): 17-20,24.
WANG Guanghuan1 CHEN Kaihong1 ZHONG Jun1 WANG Fusheng1 XU Chengbin1 JIANG Xuewu2. Effects of environmental estrogen on Notch signaling pathway in neonatal rat′s gubernaculum. 中国医药导报, 2018, 15(33): 17-20,24.

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