Experimental study on seborrheic alopecia in animals of Sanshen Yangfa Sprays
SHI Xiaofang1 LI Jian1 ZHAO Shengxia1 ZHAO Zhangguang1 SUN Jianhui2 HUO Hairu2 LI Xiaoqin2 LI Hongmei2
1.Beijing Zhangguang 101 Technology Co., Ltd., Beijing 100176, China;
2.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Abstract：Objective To investigate the therapeutic effect and anti-inflammatory effct of Sanshen Yangfa Sprays on the seborrheic alopecia animal model. Methods Sixty SPF male Wistar rats and sixty SPF male Institute of Cancer Research (ICR) mice were injected with testosterone propionate for 60 consecutive days, resulting in experimental seborrheic alopecia model. According to the weight block, the rats and mice were randomly divided into normal control group, model control group, high dose (0.290 g crude drug/mL), medium dose (0.145 g crude drug/mL), low dose (0.073 g crude drug/mL) of Sanshen Yangfa Sprays group, and Minoxidil Tincture group (0.05 g/mL), with 10 animals in each group, the length of hair was measured, the hair follicles and sebaceous glands were examinated by pathologic histology. According to the weight block, 50 SPF male C57BL/6 mice were randomly divided into blank control group, high dose (0.290 g crude drug/mL), medium dose (0.145 g crude drug/mL), low dose (0.073 g crude drug/mL) of Sanshen Yangfa Sprays group, and Minoxidil Tincture group (0.05 g/mL), with 10 mice in each group, the time of mouse hair entering the growth period from the rest period, the time from entering the growth period to growing the skin surface, and the length of hair growth were observed. The model of capillary permeability of mice induced by acetic acid and the model of mouse ear swelling induced by croton oil were used to research the anti-inflammatory effect of Sanshen Yangfa Sprays. According to weight block, 50 SPF male ICR mice were randomly divided into model control group, high dose(0.290 g crude drug/mL), medium dose (0.145 g crude drug/mL), low dose (0.073 g crude drug/mL) of Sanshen Yangfa Sprays group, and Yunnan Baiyao Tincture group, with 10 mice in each group, the absorbance of peritoneal fluid and value of ear swelling were measured. Results At the 15th, 30th, 45th, 60th day of drug administration, the hair length of rats in the high, medium and low dose of Sanshen Yangfa Sprays group were longer than those in the model control group (P < 0.05); the lesion degree of dermal tissue cells, hair follicles and sebaceous glands in rats of the high and medium dose of Sanshen Yangfa Sprays group were lower than those of the model control group (P < 0.05); the number of damaged hair follicles in the rats of the high and medium dose of Sanshen Yangfa Sprays group was lower than those of the model control group (P < 0.05). At the 15th, 30th and 45th day of administration, the hair length of the mice of the high and medium dose of Sanshen Yangfa Sprays group were longer than those of the model control group (P < 0.05); the number of damaged hair follicles in the mice of the high and medium dose of Sanshen Yangfa Sprays group were significantly lower than those of the model control group (P < 0.05); the lesion degree of dermal tissue cells, hair follicles and sebaceous glands in mice of the high dose of Sanshen Yangfa Sprays group was lower than that of the model control group (P < 0.05). The time of hair follicles from resting period to growing period, from entering growing period to growing surface of mice in high and medium dose of Sanshen Yangfa Sprays group were significantly shorter than those of blank control group (P < 0.05); the hair length was significantly higher than that of the blank control group at the 15th, 30th, 45th day of administration (P < 0.05). The absorbance of peritoneal fluid of mice in the high dose of Sanshen Yangfa Sprays group was significantly lower than that in the model control group (P < 0.05). The value of ear swelling of mice in the high and medium dose of Sanshen Yangfa Sprays group was significantly lower than that in the model control group (P < 0.01). Conclusion Sanshen Yangfa Sprays have the effect of promoting hair growth and anti-inflammatory effect on sebrrheic alopecia.
史小方1 李健1 赵胜霞1 赵章光1 孙建辉2 霍海如2 李小芹2 李洪梅2. 三参养发喷雾剂对动物脂溢性脱发的实验研究[J]. 中国医药导报, 2018, 15(32): 16-23.
SHI Xiaofang1 LI Jian1 ZHAO Shengxia1 ZHAO Zhangguang1 SUN Jianhui2 HUO Hairu2 LI Xiaoqin2 LI Hongmei2. Experimental study on seborrheic alopecia in animals of Sanshen Yangfa Sprays. 中国医药导报, 2018, 15(32): 16-23.
[1] 陈方，张生枝，陈卫琴，等.黄蜀葵对实验性脂溢性脱发的影响初探[J].赣南医学院学报，2009，29（3）：327-330.
[2] 哈斯其美格.中药“舒发康”治疗脂溢性脱发的实验研究[J].西北民族大学学报，2009，30（4）：61-67.
[3] 赵玉磊，范卫新.中药黄芪、女贞子、人参促毛发生长的在体研究[J].临床皮肤科杂志，2003，32（6）：19-20.
[4] 张焱，余勇，吴立成.二苯乙烯苷对C57BL/6J小鼠毛发生长影响的研究[J].浙江医学教育，2013，12（2）：38-41.
[5] 张春洪.生发酊对C57BL/6小鼠毛发生长作用的实验研究[J].江苏中医药，2012，44（1）：74-75.
[6] 陈奇.中药药理研究方法学[M].北京：人民卫生出版社，1993：305.
[7] 李仪奎.中药药理实验方法学[M].上海：上海科技出版社，1991：300.
[8] 林汉彪.雄激素性脱发、斑秃患者生活质量及抑郁情况调查研究[J].中国当代医药，2018，25（23）：179-181，185.
[9] Lee WS，Lee HJ. Characteristics of androgenetic alopecia in Asian [J]. Ann Dermatol，2012，24（3）：243-252.
[10] Wang TL，Zhou C，Shen YW，et al. Prevalence of androgenetic alopecia in China：a community-based study in six cities [J]. Br J Dermatol，2010，162（4）：843-847.
[11] 赵玉玲，霍青云，周芳，等.邱明义教授治疗脂溢性脱发的经验[J].环球中医药，2017，10（8）：991-993.
[12] 孙双，王育红，张芙娟，等.复方姜汁当归洗剂的临床观察与分析[J].北方药学，2013，10（2）：14-15.
[13] 莫兴群，胡金辉.祛脂生发液对56例脂溢性脱发患者头发生长自我评价水平的影响[J].医学信息，2013（11）：172-173.
[14] 吕海鹏，苏志坚，王官清，等.椒莲酊剂治疗男子雄性激素源性秃发50例[J].中医研究，2014，27（8）：14-15.
[15] Inui S，Itami S. Androgen actions on the human hair follicle：perspectives [J]. Exp Dermatol，2013，22（3）：168-171.
[16] Cotsaerlis G，Sun TT，Lavker RM. Label-retaining cells reside in the bulge area of pilosebaceous unit：implications for follicular stem cells，hair cycle，and sikn carcinogenesis [J]. Cell，1990，61：1329-1337.
[17] Stenn KS. The MoleCular and Structural Biology of Hair [J]. Ann NY Acad Sci，1991，642：xi-xiii.
[18] Hardy MH. The secret life of the hair follicle [J]. Trends Geneti，1992，8（2）：55-61.
[19] Oh HS，Smart RC. An estrogen receptor pathway regulates the telogen-anagen hair of follicle transition and influencesepidermal cell proliferation [J]. Proc Natl Sci U S A，1996，93（22）：12525-12530.
[20] Slominski A，Paus R，Costantino R. Differential expression and activity of melanogenesis-related proteins during induced hair growth in mice [J]. J Invest Dermatol，1991，96（2）：172-179.
[21] 张春洪.生发酊对C57BL/6小鼠毛囊细胞凋亡的影响[J].中国当代医药，2016，23（12）：122-124，128.
[22] Ebling FJG. The hormonal control of hair growth [C]// Orfanos CE，Happle R. Hair and hair diaeases. Berlin：Spirnger-Berlag，1990：267-299.
[23] 张国强，程毅，李玲，等.复方甘草酸苷、地塞米松对毛乳头细胞活性影响的研究[J].天津医药，2011，39（2）：127-129.
[24] 杨琛，尹中普.地塞米松不同给药方式治疗慢性分泌性中耳炎的疗效和安全性比较[J].中国药房，2017，28（21）：2919-2922.
2018, Vol. 15 
京公网安备 11010502046598号