Effects of Yutang Pills on IRS-1 gene expression and protein phosphorylation in pancreatic tissue of diabetic rats
ZHENG Nan1 XIE Ning1 ZHANG Peiqing2 GAO Lijuan3
1.Basic Medical College, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Department of Kidney Diseases, Heilongjiang Provincial Academy of Chinese Medicine, Heilongjiang Province, Harbin 150000, China;
3.Department of Rheumatology, First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China
Abstract：Objective To study the effects of Yutang Pills on gene expression of insulin receptor substrate-1 (IRS-1) and protein phosphorylation in pancreatic tissue of diabetic rats. Methods In this study, 150 SD rats were randomly divided into two groups according to their weight, with 20 rats in the blank group and 130 rats in the model group. The model group was fed with high fat forage for 10 weeks to prepare type 2 diabetic rat models and then divided into model control group, Tangniaole group, high dosage of Yutang Pills group, low dosage of Yutang Pills group and Glucophage group according to random number table method, with 20 rats in each group. With the equivalent dosage of rats and adults, the high dosage of Yutang Pills group was given 2.70 g/(kg·d) of Yutang Pills, low dosage of Yutang Pills group was given 1.35 g/(kg·d) of Yutang Pills, Tangniaole group was given 2.43 g/(kg·d) of Tangniaole, and Glucophage group was given 0.09 g/(kg·d) of Glucophage, meanwhile, the model control group and the blank group were given 2 mL/d of normal saline. The pancreatic tissues of rats were taken after 8 weeks of continuous intragastric administration, IRS-1 mRNA expression level was detected by Real-time PCR, and IRS-1 protein expression and phosphorylation levels were detected by Western blot. Results Compared with the model control group, the relative expression of IRS-1 mRNA in Glucophage group, Tangniaole group, low dosage of Yutang Pills group and high dosage of Yutang Pills group was increased (P ＜ 0.05 or P ＜ 0.01), and the relative expression of IRS-1 mRNA in high dosage of Yutang Pills group was higher than that in low dosage of Yutang Pills group (P ＜ 0.05). Compared with the model control group, the levels of IRS-1 ser307 protein phosphorylation in Glucophage group, Tangniaole group, low dosage of Yutang Pills group and high dosage of Yutang Pills group were significantly decreased (P ＜ 0.01), and the level of IRS-1 ser307 protein phosphorylation in high dosage of Yutang Pills group was lower than that in low dosage of Yutang Pills group, the difference was highly statistically significant (P ＜ 0.01). Conclusion Yutang Pills can play a therapeutic role in type 2 diabetes mellitus by affecting the relative expression of IRS-1 mRNA and inhibiting the progress of ser307 protein phosphorylation on IRS-1 to reduce insulin resistance.
2018, Vol. 15 
京公网安备 11010502046598号