细胞自噬对岩大戟内酯B诱导的白血病细胞HL-60凋亡的影响

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摘要目的探讨自噬特异性抑制剂3-甲基腺嘌呤（3-MA）和自噬激动剂雷帕霉素（RAPA）对岩大戟内酯B诱导的人白血病细胞系HL-60细胞凋亡的影响。方法采用Western blot分析检测不同浓度的岩大戟内酯B作用不同时间后LC3-Ⅱ/LC3-Ⅰ和自噬调节因子Beclin-1等的表达；将HL-60细胞分为空白对照组、岩大戟内酯B组、岩大戟内酯B联合5 mmol/L 3-MA组、岩大戟内酯B联合10 mmol/L 3-MA组、岩大戟内酯B联合10 nmol/L RAPA组和岩大戟内酯B联合20 nmol/L RAPA组。采用AnnexinV-FITC/PI双染流式细胞仪检测人白血病细胞系HL-60细胞的凋亡情况；检测Caspase-3蛋白酶的活性。结果岩大戟内酯B能够诱导白血病细胞HL-60自噬，增加LC3-Ⅱ和自噬调节因子Beclin-1的表达（P < 0.05）；3-MA可降低岩大戟内酯B诱导的HL-60细胞凋亡和Caspase-3的活性（P < 0.05），而RAPA则提高了岩大戟内酯B诱导的HL-60细胞凋亡率及Caspase-3的活性（P < 0.05）。结论岩大戟内酯B可诱导HL-60细胞自噬，细胞自噬在岩大戟内酯B诱导的HL-60细胞凋亡中起到了非常重要的作用。

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	王佳贺王超

关键词 ：岩大戟内酯B, 白血病, HL-60细胞, 细胞凋亡, 自噬

Abstract：Objective To investigate the effects of apoptosis of the autophagy-specific inhibitor 3-methyladenine (3-MA) and autophagy agonist rapamycin (RAPA) on the human leukemia cell line HL-60 cells induced by Jolkinolide B. Methods The expressions of autophagy-related protein LC3-Ⅱ/LC3-Ⅰand autophagy regulator Beclin-1 induced by different doses of Jolkinolide B at different time points were detected by Western blot method. The HL-60 cells were divided into blank control group, Jolkinolide B group, Jolkinolide B combined with 5 mmol/L 3-MA group, Jolkinolide B combined with 10 mmol/L 3-MA group, Jolkinolide B combined with 10 nmol/L RAPA group and Jolkinolide B combined with 20 nmol/L RAPA group. Apoptosis of human leukemia cell line HL-60 cells was detected by Annexin V-FITC/PI double staining flow cytometry. Moreover, the activity of Caspase-3 was detected. Results Jolkinolide B could induce autophagy in leukemia cell line HL-60, increase the expression of LC3-Ⅱ and autophagy regulator Beclin-1 (P < 0.05). 3-MA could lower the apoptosis rate of HL-60 cells and the activity of Caspase-3 activity induced by Jolkinolide B (P < 0.05), while RAPA increased the apoptosis rate of HL-60 cells and the activity of Caspase-3 induced by Jolkinolide B (P < 0.05). Conclusion Jolkinolide B can induce autophagy in HL-60 cells, and autophagy plays an important role in the apoptosis of HL-60 cells induced by Jolkinolide B.

Key words： Jolkinolide B Leukemia HL-60 cell Apoptosis Autophagy

基金资助:辽宁省自然科学基金指导计划项目（201602858）。

引用本文:

王佳贺王超. 细胞自噬对岩大戟内酯B诱导的白血病细胞HL-60凋亡的影响[J]. 中国医药导报, 2018, 15(28): 13-16细胞自噬对岩大戟内酯B诱导的.
WANG Jiahe WANG Chao. Effect of cell autophagy on apoptosis of HL-60 cells induced by Jolkinolide B. 中国医药导报, 2018, 15(28): 13-16细胞自噬对岩大戟内酯B诱导的.

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