Abstract：Objective To develop hydroxyapatite functionalized multi-walled carbon nanotubes (HA-MWCNTs), and assess the toxicity of HA-MWCNTs on rat′s BMSCs and its influence on the multipotent differentiation of bone marrow derived mesenchymal stem cells (BMSCs), thereby to provide experimental evidence for its utility in tissue engineering. Methods HA-MWCNTs were synthesized. Transmission electron microscope (TEM), X-ray diffraction (XRD) were used to observe the microstructure; BMSCs were obtained from female SD rats of 4 weeks old which weighted between 100 to 150 grams. After cultured and expanded to the third passage, cells were co-cultured with HA-MWCNTs and unfunctionalized MWCNTs (MWCNTs). MTT and live-dead cell assay were used to appraise the toxicity of MWCNTs on BMSCs. BMSCs were then induced and differentiated into adipocytic and osteoblastic cells and were used to assess the influence of MWCNTs on BMSCs′ multipotent differentiation. Results Compared to control group, BMSCs in MWCNTs group were significantly inhibited (P < 0.05), while in HA-MWCNTs group, BMSCs were slightly inhibited, with no statistical difference (P > 0.05), and the ratio of dead cells was less in HA-MWCNTs group. The mRNA level of osteogenesis (Cbfal/Runx2, BSP and OCN) was increased (P < 0.05) and the mRNA of adipogenesis (aP2 and LPL) was decreased (P < 0.05) in HA-MWCNTs group. Conclusion Compared with MWCNTs, HA-MWCNTs had slight influence on cell proliferation, trivial toxicity and favorable biocompatibility of BMSCs, meanwhile it could promote osteoblastic and inhibit adipocytic differentiation of BMSCs. HA-MWCNTs may supply a novel platform for applications of scaffold in bone tissue engineering.
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2018, Vol. 15 
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