Abstract：Objective To observe the effect of nitric oxide on formation of deprivation myopia (FDM) in guinea pig, in order to investigate the regulation of nitric oxide on retinopathy of myopia. Methods Guinea pigs were used to establish the FDM model. They were divided into group A (FDM-untreated), group B (FDM induced 10-day), group C (FDM induced 20-day), group D (FDM induced 10-day plus NOS inhibitor injected), group E (FDM induced 20-day plus NOS inhibitor injected), group F (FDM induced 10-day plus melatonin injected), group G (FDM induced 20-day plus melatonin injected), with 15 guinea pigs in each group. The myopic refraction, axial length in each group and expression level of iNOS in the retina of guinea pigs were observed. Results Compared to group A, myopia increased and the axial length prolonged significantly, and the level of iNOS increased obviously in FDM-induced group, with statistically significant difference (P < 0.05). Compared to FDM-induced group, the level of iNOS significantly reduced (P < 0.05), and the degree of myopia and the axial length were severely inhibited in guinea pigs treated with FDM plus NOS inhibitor injected. Conclusion The expression of iNOS in retina in FDM guinea pigs increases, while NOS inhibitor can effectively inhibite the occurrence of myopia. The synthesis of nitric oxide promotes FDM in guinea pig.
[1] Pararajasegaram R. VISION 2020-the right to sight：from strategies to action [J]. Am J Ophthalmol，1999，128（3）：359-360.
[2] Wallman J，Winawer J. Homeostasis of eye growth and the question of myopia [J]. Neuron，2004，43（4）：447-468.
[3] Wiesel TN，Raviola E. Myopia and eye enlargement after neonatal lid fusion in monkeys [J]. Nature，1977，266（5597）：66-68.
[4] Wu XH，Li YY，Zhang PP，et al. Unaltered retinal dopamine levels in a C57BL/6 mouse model of form-deprivation myopia [J]. Invest Ophthalmol Vis Sci，2015，56（2）：967-977.
[5] Mao J，Liu S. Different roles of retinal dopamine in albino guinea pig myopia [J]. Neurosci Lett，2017，639：94-97.
[6] Mathis U，Ziemssen F，Schaeffel F. Effects of a human VEGF antibody（Bevacizumab）on deprivation myopia and choroidal thickness in the chicken [J]. Exp Eye Res，2014， 127：161-169.
[7] Hoshi H，Sato M，Oguri M，et al. In vivo nitric oxide concentration in the vitreous of rat eye [J]. Neurosci Lett，2003， 347（3）：187-190.
[8] Westermann BA，Meissl H. Nitric oxide synthase in photoreceptive pineal organs of fish [J]. Comp Biochem Physiol A Mol Integr Physiol，2008，151（2）：198-204.
[9] Shi Q，Stell WK. Die Fledermaus：regarding optokinetic contrast sensitivity and light-adaptation，chicks are mice with wings [J]. PLoS One，2013，8（9）：e75375.
[10] An J，Hsi E，Zhou X，et al. The FGF2 gene in a myopia animal model and human subjects [J]. Mol Vis，2012，18：471-478.
[11] Maddox JW，Gleason E. Nitric oxide promotes GABA release by activating a voltage-independent Ca2+ influx pathway in retinal amacrine cells [J]. J Neurophysiol，2017，117（3）：1185-1199.
[12] Nickla DL，Wilken E，Lytle G，et al. Inhibiting the transient choroidal thickening response using the nitric oxide synthase inhibitor l-NAME prevents the ameliorative effects of visual experience on ocular growth in two different visual paradigms [J]. Exp Eye Res，2006，83（2）：456-464.
[13] Chiou GC. Review：effects of nitric oxide on eye diseases and their treatment [J]. J Ocul Pharmacol Ther，2001，17（2）：189-198.
[14] Iwakiri Y. Nitric oxide in liver fibrosis：the role of inducible nitric oxide synthase [J]. Clin Mol Hepatol，2015， 21（4）：319-325.
[15] Zhang B，Crankshaw W，Nesemeier R，et al. Calcium-mediated signaling and calmodulin-dependent kinase regulate hepatocyte-inducible nitric oxide synthase expression [J]. J Surg Res，2015，193（2）：795-801.
[16] Stone RA，Pardue MT，Iuvone PM，et al. Pharmacology of myopia and potential role for intrinsic retinal circadian rhythms [J]. Exp Eye Res，2013，114：35-47.
[17] Blanco S，Hernandez R，Franchelli G，et al. Melatonin influences NO/NOS pathway and reduces oxidative and nitrosative stress in a model of hypoxic-ischemic brain damage [J]. Nitric Oxide，2017，62：32-43.
[18] Moore WM，Webber RK，Jerome GM，et al. L-N6-（1-iminoethyl）lysine：a selective inhibitor of inducible nitric oxide synthase [J]. J Med Chem，1994，37（23）：3886-3888.
[19] Yang Y，Zhang J，Liu Y，et al. Role of nitric oxide synthase in the development of bone cancer pain and effect of L-NMMA [J]. Mol Med Rep，2016，13（2）：1220-1226.
[20] Radomski MW，Jenkins DC，Holmes L，et al. Human colorectal adenocarcinoma cells：differential nitric oxide synthesis determines their ability to aggregate platelets [J]. Cancer Res，1991，51（22）：6073-6078.
2017, Vol. 14 
京公网安备 11010502046598号