Efects of Eucommia ulmoides oliver flavones on apoptotic of pediatric hemangioma endothelial cells
LI Yang1 LIU Guoliang2 YAO Yuan2 ZHANG Ning2▲ GUO Wei3▲
1.Department of Pediatric Surgery, Jinzhou Maternity and Infant Hospital, Liaoning Province, Jinzhou 121000, China;
2.College of Jiamusi, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China;
3.The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150041, China
Abstract：Objective To study the influence of Eucommia ulmoides oliver flavones (EUOF) on apoptosis of infantile hemangioma endothelial cell. Methods Infantile hemangioma endothelial cells were cultured in vitro. The cells was divided into blank groups (normal cultivate infantile hemangioma endothelial cells), high dose group of EUOF (50 μg/mL EUOF treatment infantile hemangioma endothelial cells), the middle dose group (5 μg/mL EUOF treatment infantile hemangioma endothelial cells), the low dose group (0.5 μg/mL EUOF treatment children hemangioma endothelial cells), then cultured for 72 hours, MTT method detected cell vitality. The TUNEL method detected apoptosis of cells; Western blot method detected expression levels of apoptosis related proteins Bax, Bcl-2 and caspase-9 in infantile hemangioma endothelial cell. Results Compared to the blank group, infantile hemangioma endothelial cell proliferation could inhibit in EUOF middle dose group, high dose group, the differences were statistically significant (P < 0.05). The rate of apoptosis in high dosages group and middle dosages group rose, the differences were statistically significant (P < 0.05); the expression of Bax in high dose group rose, differences were statistically significant (P < 0.01). The expression of caspase-9 potein in the high dose group middle dose group and low dose group rose, the differences were statistically significant (P < 0.05). The expression of bcl-2 in high dose group, middle dose group decreased, the differences were statistically significant (P < 0.05). Conclusion EUOF can inhibit the the expression of Bcl-2 protein and promote the expression of Bax and caspase-9 in infantile hemangioma endothelial cells, that is expected to be an effective therapeutic agent in the disease of hemangioma.
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