Study on the effect of Xiaotan Sanjie Recipe on apoptosis of colon cancer stem-like cells and its related mechanism
ZHOU Yuqi1,2 ZHANG Yingcheng1 WEI Pinkang1▲ YE Min1▲
1.Department of Traditional Chinese Medicine, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China;
2.Department of Hematology and Oncology, the 101st Hospital of People's Liberation Army, Jiangsu Province, Wuxi 214044, China
Abstract：Objective To investigate effect of Xiaotan Sanjie Recipe on apoptosis of colon cancer stem-like cells (CCSCs) and its related mechanism. Methods The CCSCs were induced from colon cancer cell line HCTl16 in serum-free medium. The expressions of CD44 were detected to identify CCSCs. The lyophilized powder of Xiaotan Sanjie Recipe Extract was dissolved in cell culture fluid, so as to make preserving fluid of Xiaotan Sanjie Recipe. CCSCs were treated with different concentrations of Xiaotan Sanjie Recipe (0.125, 0.25, 0.5, 1.0 mg/mL) for 72 h. The effects of different concentrations of Xiaotan Sanjie Recipe for CCSCs were detected by Annexin-V/PI staining combined with flow cytometry. The expression changes of Bax, Bcl-2 protein after drug intervention were detected by Western blot. Results HCT116 cells formed cancer stem cell spheres in serum-free medium. The proportion of CD44 cells in cell spheres was (49.69±1.89)%. Xiaotan Sanjie Recipe could induce the cell apoptosis, the apoptosis rate was statistically significant different compared with the control group (0 mg/mL) (P ＜ 0.05), the apoptotic effects showed concentration-dependent. After application of Xiaotan Sanjie Recipe, the expression of pro-apoptotic protein Bax of Bcl-2 family was increased, meanwhile the expression of anti-apoptotic protein Bcl-2 was decreased. The effect was more pronounced in 0.5 mg/mL and 1.0 mg/mL concentration groups. Conclusion CCSCs are successfully induced from HCT116 colon cancer cell line. Xiaotan Sanjie Recipe can induce CCSCs in a dose-dependent manner, the mechanism may be related to the increased expression of pro-apoptotic protein Bax and decreased expression of anti-apoptotic protein Bcl-2.
周昱岐1，2 张映城1 魏品康1▲ 叶敏1▲. 消痰散结方对结肠癌干细胞样细胞凋亡的影响及相关机制研究[J]. 中国医药导报, 2017, 14(24): 17-20.
ZHOU Yuqi1,2 ZHANG Yingcheng1 WEI Pinkang1▲ YE Min1▲. Study on the effect of Xiaotan Sanjie Recipe on apoptosis of colon cancer stem-like cells and its related mechanism. 中国医药导报, 2017, 14(24): 17-20.
[1] Vaiopoulos AG，Kostakis ID，Koutsilieris M，et al. Concise Review：Colorectal Cancer Stem Cells [J]. Stem Cells，2012， 30（3）：363-371.
[2] 董志伟，乔友林，李连弟，等.中国癌症控制策略研究报告[J].中国肿瘤，2002，11（5）：250-260.
[3] Abdul Khalek FJ，Gallicano GI，Mishra L. Colon cancer stem cells [J]. Gastrointest Cancer Res，2010（Suppl 1）：S16-S23.
[4] Paldino E，Tesori V，Casalbore P，et al. Tumor initiating cells and chemoresistance：which is the best strategy to target colon cancer stem cells? [J] Biomed Res Int，2014， 2014：859871.
[5] de Sousa EM，Vermeulen L，Richel D，et al. Targeting Wnt signaling in colon cancer stem cells [J]. Clin Cancer Res，2011，17（4）：647-653.
[6] Li YF，Xiao B，Tu SF，et al. Cultivation and identification of colon cancer stem cell-derived spheres from the Colo205 cell line [J]. Braz J Med Biol Res，2012，45（3）：197-204.
[7] 武峰，秦志丰，张慈安，等.金龙蛇颗粒对胃癌术后患者生存质量的影响[J].中国中西医结合消化杂志，2012，7（20）：289-292.
[8] Sun DZ，Jiao JP，Zhang X，et al. Therapeutic effect of Jinlongshe Granule on quality of life of stage Ⅳ gastric cancer patients using EORTC QLQ-C30：A double-blind placebo-controlled clinical trial [J]. Chin J Integr Med，2016， 21（8）：579-586.
[9] Yao CJ，Yeh CT，Lee LM，et al. Elimination of cancer stem-like“side population” cells in hepatoma cell lines by Chinese herbal mixture “tien-hsien liquid” [J]. Evid Based Complement Alternat Med，2012，2012：617085.
[10] 唐琪琳，杨帆，王学岭.大肠癌的中医病因病机及治疗研究概况[J].长春中医药大学学报，2016，32（1）：216-219.
[11] 郭飘婷，王松坡.历代中医论治大肠癌[J].吉林中医药，2016，36（3）：223-227.
[12] 周昱岐，叶敏，吕灿，等.消痰散结方对人结直肠癌HCT116细胞增殖、凋亡和Caspase3蛋白表达的影响[J].中国中医药信息杂志，2015，22（3）：60-64.
[13] 周昱岐，张映城，魏品康，等.消痰散结方体外诱导人结直肠癌HCT116细胞周期阻滞的作用及相关机制研究[J].上海中医药杂志，2016，50（2）：74-77.
[14] Weiswald LB，Bellet D，Dangles-Marie V. Spherical cancer models in tumor biology [J]. Neoplasia，2015，17（1）：1-15.
[15] Mather JP. In vitro models [J]. Stem Cells，2012，30（2）：95-99.
[16] Feng F，Jiang Y，Lu H，et al. Rab27A mediated by NF-κB promotes the stemness of colon cancer cells via up-regulation of cytokine secretion [J]. Oncotarget，2016，7（39）：63342-63351.
[17] Finlay D，Teriete P，Vamos M，et al. Inducing death in tumor cells：roles of the inhibitor of apoptosis proteins [J]. F1000Res，2017，6：587
[18] Birkinshaw RW，Czabotar PE. The BCL-2 family of proteins and mitochondrial outer membrane permeabilisation [J]. Semin Cell Dev Biol，2017. [Epub ahead of print]
[19] Vadde R，Radhakrishnan S，Reddivari L，et al. Triphala Extract Suppresses Proliferation and Induces Apoptosis in Human Colon Cancer Stem Cells via Suppressing c-Myc/Cyclin D1 and Elevation of Bax/Bcl-2 Ratio [J]. Biomed Res Int，2015，2015：649263.
[20] Reddivari L，Charepalli V，Radhakrishnan S，et al. Grape compounds suppress colon cancer stem cells in vitro and in a rodent model of colon carcinogenesis [J]. BMC Complement Altern Med，2016，9（16）：278.
2017, Vol. 14 
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