Abstract：Objective To investigate the changes of Antibiotic resistance of Stenotrophomonas maltophilia and the expression of efflux pump SmeDEF in the induction of Triclosan. Methods From March to June in 2017, 10 strains of Stenotrophomonas maltophilia isolated from bacterial lab cultured in Weifang People′s Hospital were selected. The MIC of Stenotrophomonas maltophilia before and after the induction of Triclosan was compared and the repression level of the mRNA of efflux pump smeD was monitored by RT-PCR. Results The inhibition of 10 strains of bacteria pump sensitive to antibiotics was positive or negative. The levofloxacin MIC of 6 strains was 3-5 dilution higher than that before induction, and the MIC of 6 strains was reduced when the efflux pump inhibitor CCCP was used. The repression level of smeD of both pump inhibitor positive strains antibiotic overnight group and logarithmic growth addition group was significantly higher the no antibiotic group. Conclusion The resistance to antibiotics of Stenotrophomonas maltophilia clinical isolates induced by triclosan has increased, which is related to the expression of efflux pump SmeDEF.
高瑞灏1 王有森1 刘德美2▲. 三氯生在嗜麦芽窄食单胞菌耐药机制中的作用[J]. 中国医药导报, 2018, 15(23): 113-116.
GAO Ruihao1 WANG Yousen1 LIU Demei2▲. The effect of Triclosan on the resistance mechanism of Stenotrophomonas maltophilia. 中国医药导报, 2018, 15(23): 113-116.
[1] Aiello AE，Larson EL，Levy SB. Consumer antibacterial so-aps：effective or just risky [J]. Clin Infect Dis，2007，45：S137-147.
[2] Muynck WD，Belie ND，Verstraete W. Antimicrobial mortar surfaces for the improvement of hygienic conditions [J]. J Appl Microbiol，2010，108（1）：62-72.
[3] Moretro T，Sonerud T，Mangelrod E，et al. Evaluation of the antibacterial effect of a triclosan-containing floor used in the food industry [J]. J Food Prot，2006，69（3）：627-633.
[4] Jones RD，Jampani HB，Newman JL，et al. Triclosan：a review of effectiveness and safety in health care settings [J]. Am J Infect Control，2000，28（2）：184-196.
[5] Sjoblom M，Ainamo A，Ainamo J. Antimicrobial effect of four different toothpastes [J]. Scand J Dent Res，1979，84（6）：377-380.
[6] Bhargava HN，Leonard PA. Triclosan：applications and safety [J]. Am J Infect Control，1996，24（3）：209-218.
[7] Meyer B，Cookson B. Does microbial resistance or adaptation to biocides create a hazard in infection prevention and control [J]. J Hosp Infect，2010，76（3）：200-205.
[8] Aiello AE，Larson E. Antibacterial cleaning and hygiene products as an emerging risk factor for antibiotic resistance in the community [J]. Lancet Infect Dis，2003，3（8）：501-506.
[9] Levy SB. Antibacterial household products：cause for concern [J]. Emerg Infect Dis，2001，7：512-515.
[10] Levy SB. Active efflux，a common mechanism for biocide and antibiotic resistance [J]. Symp Ser Soc Appl Microbiol，2002（31）：65S-71S.
[11] Sanchez P，Moreno E，Martinez JL. The biocide triclosan selects Stenotrophomonas maltophilia mutants that overproduce the SmeDEF multidrug efflux pump [J]. Antimicrob Agents Chemother，2005，49（2）：781-782.
[12] Chuanchuen R，Beinlich K，Hoang TT，et al. Cross-resistance between triclosan and antibiotics in Pseudomonas aeruginosa is mediated by multidrug efflux pumps：exposure of a susceptible mutant strain to triclosan selectsnfxB mutants overexpressing MexCD-OprJ [J]. Antimicrob Agents Chemother，2001，45（2）：428-432.
[13] McMurry LM，Oethinger M，Levy SB. Overexpression of marA，soxS，or acrAB produces resistance to triclosan in laboratory and clinical strains of Escherichia coli [J]. FEMS Microbiol Lett，1998，166（2）：305-309.
[14] 岳玮，谭薇，李晓明.抗生素诱导嗜麦芽窄食单胞菌临床株外排泵SmeDEF表达的研究[J].潍坊医学院学报，2013，35（2）：100-103.
[15] Pumbwe L，Chang A，Smith RL，et al. Clinical significance of overexpression of multiple RND-family efflux pumps inBacteroides fragilis isolates [J]. J Antimicrob Chemother，2006，58（3）：543-548.
[16] Cottell A，Denyer SP，Hanlon GW，et al. Triclosan tolerant bacteria：changes in susceptibility to antibiotics [J]. J Hosp Infect，2009，72（1）：71-76.
[17] Martinez JL，Sanchez MB，Martinez-Solano L，et al. Functional role of bacterial multidrug efflux pumps in microbial natural ecosystems [J]. FEMS Microbiol Rev，2009， 33（2）：430-449.
[18] Looney WJ，Narita M，Muhlemann K. Stenotrophomonas maltophilia：an emerging opportunist human pathogen [J]. Lancet Infect Dis，2009，9（5）：312-323.
[19] Cho SY，Lee DG3，Choi SM，et al. Stenotrophomonas maltophilia bloodstream infection in patients with hematologic malignancies： a retrospective study and in vitro activities of antimicrobial combinations [J]. BMC Infect Dis，2015，18（15）：69.
[20] Subhani S，Patnaik AN，Barik R，et al. Infective endocarditis caused by Stenotrophomonas maltophilia：a report of two cases and review of literature [J]. Indian Heart J，2016，68（2）：S267-S270.
[21] Turrientes MC，Baquero MR，Sanchez MB，et al. Polymorphic mutation frequencies of clinical and environmental Stenotrophomonas maltophilia populations [J]. Appl Environ Microbiol，2010，76（6）：1746-1758.
[22] Ryan RP，Monchy S，Cardinale M，et al. The versatility and adaptation of bacteria from the genus Stenotrophom-onas [J]. NatRev Micro，2009，7（7）：514-525.
[23] Hernandez A，Mate MJ，Sanchez-Diaz PC，et al. Structural and Functional Analysis of SmeT，the Repressor of the Stenotrophomonas maltophilia Multidrug Efflux Pump SmeDEF [J]. J Biol Chem，2009，284（21）：14 428-14 438.
[24] Sanchez P，Alonso A，Martinez JL. Cloning and characterization of SmeT，a repressor of the Stenotrophomonas maltophilia multidrug efflux pump SmeDEF [J]. Antimicrob Agents Chemother，2002，46（11）：3386-3393.
[25] Ramos JL，Martinez-Bueno M，Molina-Henares AJ，et al. The TetR family of transcriptional repressors [J]. Microbiol Mol Biol Rev，2005，69（2）：326-356.
[26] Natsume R，Ohnishi Y，Senda T，et al. Crystal structure of a gamma-butyrolactone autoregulator receptor protein in Streptomyces coelicolor A3（2） [J]. J Mol Biol，2004，13， 336（2）：409-419.
[27] Dover LG，Corsino PE，Daniels IR，et al. Crystal structure of the TetR/CamR family repressor Mycobacterium tuberculosis EthR implicated in ethionamide resistance [J]. J Mol Biol，2004，340（5）：1095-1105.
[28] Gu R，Su CC，Shi F，et al. Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni [J]. J Mol Biol，2007，372（3）：583-593.
[29] Li M，Gu R，Su CC，et al. Crystal structure of the transcriptional regulator AcrR from Escherichia coli [J]. J Mol Biol，2007，374（3）：591-603.
[30] Willems AR，Tahlan K，Taguchi T，et al. Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor（S）-DNPA [J]. J Mol Biol，2008，376（5）：1377-1387.
[31] Jeng WY，Ko TP，Liu CI，et al. Crystal structure of IcaR，a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis [J]. Nucleic Acids Res，2008，36（5）：1567-1577.
[32] Grkovic S，Brown MH，Skurray RA. Regulation of bacterial drug export systems [J]. Microbiol Mol Biol Rev，2002， 66（4）：671-701.
[33] Bailey AM，Constantinidou C，Ivens A，et al. Exposure of Escherichia coliand Salmonella enterica serovar Typhimurium to triclosan induces a species-specific response，including drug detoxification [J]. J Antimicrob Chemother，2009，64（5）：973-985.
[34] Sánchez P，Linares JF，Ruiz-Díez B.et al. Fitness of in vitro selected Pseudomonas aeruginosa nalB and nfxB multidrug resistant mutants [J]. J Antimicrob Chemother，2002，50（5）：657-664.
[35] Cole EC，Addison RM，Rubino JR，et al. Investigation of antibiotic and antibacterial agent cross-resistance in target bacteria from homes of antibacterial product users and nonusers [J]. J Appl Microbiol，2003，95（4）：664-676
2018, Vol. 15 
京公网安备 11010502046598号