裸花紫珠提取物对银屑病作用及机制研究

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摘要目的探讨裸花紫珠提取物抗银屑病作用。方法 60只KM小鼠按照随机数字表法分为模型组、裸花紫珠提取物低剂量组、裸花紫珠提取物中剂量组、裸花紫珠提取物高剂量组和阳性药对照组，每组各12只。分别灌胃给予生理盐水，0.6、1.2、2.4 g/kg裸花紫珠提取物，1 mg/kg甲氨蝶呤，连续7 d。HE法检测完整颗粒层鳞片数；Western blot技术检测Caspase3、Cleaved-Caspase3、Bax、BCL-2蛋白表达；Real-time PCR技术检测Caspase3、Bax、BCL-2基因mRNA表达。结果裸花紫珠中、高剂量组鼠尾完整颗粒层鳞片数明显高于模型组（P < 0.05）；裸花紫珠中、高剂量组促凋亡蛋白Caspase3、Cleaved-Caspase3、Bax蛋白和mRNA表达均高于模型组（P < 0.05），抗凋亡蛋白BCL-2蛋白和mRNA表达下调（P < 0.05）。结论裸花紫珠提取物通过诱导凋亡发挥抗银屑病作用。

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	巴立娜

关键词 ：裸花紫珠, 银屑病, 颗粒层, 凋亡

Abstract：Objective To determine the anti-psoriasis effect of Callicarpa nudiflora extract. Methods Sixty KM mouses were randomly divided into 5 groups by random number table (n = 12), including model group, Callicarpa nudiflora low-dose group, Callicarpa nudiflora middle-dose group, Callicarpa nudiflora high-dose group and positive control group. They were given to normal saline, Callicarpa nudiflora extract (0.6 g/kg or 1.2 g/kg or 2.4 g/kg) and 1 mg/kg methotrexate by gastric perfusion for seven days respectively. Lateral line scales of granular layer were detected by HE method. The expression of Caspase3, Cleaved-Caspase3, Bax and BCL-2 mRNA and protein were detected by Real-time PCR and Western blot. Results Lateral line scales of granular layer of Callicarpa nudiflora middle-dose and high-dose groups was increased than those in the model group (P < 0.05). The expression of the pro-apoptotic proteins Caspase3, Cleaved-Caspase3, Bax mRNA and protein in callicarpa nudiflora middle-dose and high-dose groups were up-regulated than those in the model group (P < 0.05), the expression of the anti-apoptotic protein BCL-2 mRNA and protein was down-regulated (P < 0.05). Conclusion The Callicarpa nudiflora extract can exert anti-psoriatic effect by inducing apoptosis.

Key words： Callicarpa nudiflora Psoriasis Granular layer Apoptotic

基金资助:黑龙江省自然科学基金面上项目（H2015108）。

引用本文:

巴立娜. 裸花紫珠提取物对银屑病作用及机制研究[J]. 中国医药导报, 2018, 15(21): 14-18.
BA Li′na . Study of effect and potential mechanism of Callicarpa nudiflora extract against psoriasis. 中国医药导报, 2018, 15(21): 14-18.

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