Relationship between expression of STAT5B and seizure occurrence in anaplastic gliomas
FENG Enshan1 LIANG Tingyu1 WANG Fang1 TANG Kai2
1.Department of Neurosurgery, Beijing Ditan Hospital, Capital Medical University, Beijing 100000, China;
2.Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
Abstract：Objective To identify the genes associated with the onset of seizure caused by anaplastic gliomas. Methods TCGA RNA-seq set from TCGA website was downloaded, there were 243 anaplastic glioma samples, the initial symptom of 119 cases was seizure and the initial symptom of 124 ones was not seizure. t test was performed to select differential genes between two groups. CGGA RNA-seq set was used for validation. The prognosis results were attained from Kaplan-Meier curves. Results In TCGA RNA-seq set, prognosis of patients with seizure was better than those without seizure(P = 0.04). 902 genes whose expression were discriminating between two groups. Through DAVID analysis of 902 genes, it showed that the pathway of regulation of multicellular organism growth including seven genes (STAT5B, AFG3L2, FTO, APP, GDF5, MBD5, PIK3CA) was different. simplify gene number, CGGA RNA-seq set was used for reducing gene number. Then STAT5B was left. But it could not affect prognosis of anaplastic gliomas. Conclusion In anaplastic gliomas, patients with seizure have better prognosis than those without seizure. The higher STAT5B expression, the lower frequency of seizure. The expression of STAT5B may be one of factors influencing seizure occurrence.
[1] Chen B，Liang T，Yang P，et al. Classifying lower grade glioma cases according to whole genome gene expression [J]. Oncotarget，2016，7（45）：74031-74042.
[2] Jiang T，Mao Y，Ma W，et al. CGCG clinical practice guidelines for the management of adult diffuse gliomas [J]. Cancer Lett，2016，375（2）：263-273.
[3] Dolecek TA，Propp JM，Stroup NE，et al. CBTRUS statistical report： primary brain and central nervous system tumors diagnosed in the United States in 2005-2009 [J]. Neuro Oncol，2012，14（Suppl 5）：v1-v49.
[4] Louis DN，Ohgaki H，Wiestler OD，et al. The 2007 WHO classification of tumours of the central nervous system [J]. Acta Neuropathol，2007，114（2）：97-109.
[5] Yang P，Wang Y，Peng X，et al. Management and survival rates in patients with glioma in China （2004-2010）：a retrospective study from a single-institution [J]. J Neurooncol，2013，113（2）：259-266.
[6] Chang EF，Potts MB，Keles GE，et al. Seizure characteristics and control following resection in 332 patients with low-grade gliomas [J]. J Neurosurg，2008，108（2）：227-235.
[7] Fried I，Kim JH，Spencer DD. Limbic and neocortical gliomas associated with intractable seizures：a distinct clinicopathological group [J]. Neurosurgery，1994，34（5）：815-23， 23-24.
[8] Klein M，Engelberts NH，van der Ploeg HM，et al. Epilepsy in low-grade gliomas： the impact on cognitive function and quality of life [J]. Ann Neurol，2003，54（4）：514-520.
[9] Sheth RD. Adolescent issues in epilepsy [J]. J Child Neurol，2002，17（Suppl 2）：2S23-2S27.
[10] Taphoorn MJ，Klein M. Cognitive deficits in adult patients with brain tumours [J]. Lancet Neurol，2004，3（3）：159-168.
[11] Chaichana KL，Parker SL，Olivi A，et al. Long-term seizure outcomes in adult patients undergoing primary resection of malignant brain astrocytomas [J]. Clin J Neurosurg，2009，111（2）：282-292.
[12] You G，Sha Z，Jiang T. The pathogenesis of tumor-related epilepsy and its implications for clinical treatment [J]. Seizure，2012，21（3）：153-9.
[13] Yang P，Liang T，Zhang C，et al. Clinicopathological factors predictive of postoperative seizures in patients with gliomas [J]. Seizure，2016，35：93-99.
[14] Yang P，You G，Zhang W，et al. Correlation of preoperative seizures with clinicopathological factors and prognosis in anaplastic gliomas： a report of 198 patients from China [J]. Seizure，2014，23（10）：844-851.
[15] Zhong Z，Wang Z，Wang Y，et al. IDH1/2 mutation is associated with seizure as an initial symptom in low-grade glioma： A report of 311 Chinese adult glioma patients [J]. Epilepsy Res，2015，109：100-105.
[16] Chen H，Judkins J，Thomas C，et al. Mutant IDH1 and seizures in patients with glioma [J]. Neurology，2017，88（19）：1805-1813.
[17] Fan X，Wang YY，Zhang CB，et al. Expression of RINT1 predicts seizure occurrence and outcomes in patients with low-grade gliomas [J]. J Cancer Res Clin Oncol，2015， 141（4）：729-734.
[18] Campo Verde Arbocco F，Persia FA，Persia FA，et al. Hypothyroidism decreases JAK/STAT signaling pathway in lactating rat mammary gland [J]. Mol Cell Endocrinol，2017， 450：14-23.
[19] Mishra NK，Guda C. Genome-wide DNA methylation analysis reveals molecular subtypes of pancreatic cancer [J]. Oncotarget，2017，8（17）：28990-29012.
[20] Elumalai N，Berg A，Rubner S，et al. Rational development of Stafib-2：a selective，nanomolar inhibitor of the transcription factor STAT5b [J]. Sci Rep，2017，7（1）：819.
2018, Vol. 15 
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