ATF4过表达对HepG2细胞凋亡的影响及其机制

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摘要目的观察转录激活因子4（ATF4）过表达对人肝癌HepG2细胞凋亡的影响，并探讨其机制。方法构建ATF4表达质粒，采用脂质体瞬时转染HepG2细胞。实验分为空白对照组、空质粒组和ATF4质粒组。采用流式细胞术检测细胞凋亡率，检测HepG2细胞培养液中乳酸含量，Western blot检测Warburg效应蛋白M2型丙酮酸激酶（PKM2）的表达。结果与空质粒组比较，ATF4质粒组HepG2细胞凋亡率显著增加，细胞培养液中乳酸含量显著降低，HepG2细胞中PKM2蛋白的表达显著下调（均P ＜ 0.05）。结论过表达ATF4促进HepG2细胞的凋亡，其机制可能与抑制Warburg效应有关。

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	肖婷焱1 段无暇1 许紫薇1 周寿红2 曾斌1

关键词 ：肝细胞性肝癌, 细胞凋亡, 转录激活因子4, Warburg效应, M2型丙酮酸激酶

Abstract：Objective To observe the effect of overexpression of activating transcription factor 4 (ATF4) on the apoptosis of HepG2 cells and explore the mechanism. Methods ATF4 expression plasmid was built. Hepatocellular carcinoma line HepG2 cells were transfected with ATF4 expression plasmid through liposome. The plasmids were divided into blank control group, empty plasmid group and ATF4 plasmid group. The apoptosis rate was detected by flow cytometry. The content of lactate in cell culture medium was tested. The expression of Warburg effect protein pyruvate kinase subtype M2 (PKM2) was measured by Western blot. Results Compared with the empty plasmid group, the apoptosis rate of HepG2 cells increased significantly, the content of lactate in cell culture medium decreased significantly and the expression of PKM2 decreased significantly (all P ＜ 0.05). Conclusion Overexpression of ATF4 promotes the apoptosis of hepatocellular carcinoma line HepG2 cells, the mechanism may be related to inhibition of Warburg effect.

Key words： Hepatocellular carcinoma Apoptosis Activating transcription factor 4 Warburg effect Pyruvate kinase subtype M2

基金资助:湖南省自然科学基金资助项目（08JJ3032）；
湖南省卫生厅科研计划项目（B2007100）。

通讯作者: 曾斌（1969.12-），男，博士，主任医师，副教授；研究方向：肝癌的发病机制。周寿红（1976.9-），男，博士，副教授；研究方向：神经退行性疾病及肝纤维化的防治。

作者简介: 肖婷焱（1991.1-），女，硕士；研究方向：肝癌的发病机制。

引用本文:

肖婷焱1 段无暇1 许紫薇1 周寿红2 曾斌1. ATF4过表达对HepG2细胞凋亡的影响及其机制[J]. 中国医药导报, 2018, 15(19): 12-15,29.
XIAO Tingyan1 DUAN Wuxia1 XU Ziwei1 ZHOU Shouhong2 ZENG Bin1. Effect and mechanism of overexpression of ATF4 on the apoptosis of HepG2 cells. 中国医药导报, 2018, 15(19): 12-15,29.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2018/V15/I19/12

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