The role of Hepatic insulin resistance in the pathogenesis of spontaneously diabetic OLETF rats
LI Jia1 XUE Yaoming2 PAN Yonghua2 ZHU Bo2 ZHANG Yan2
1.Department of Endocrinology, General Hospital of Guangzhou Military Command of PLA, Guangdong Province, Guangzhou 510010, China;
2.Department of Endocrinology and Metabolism, Nanfang Hospital, Nanfang Medical University, Guangdong Province, Guangzhou 510515, China
Abstract：Objective To study the effects of hepatic insulin resistance, including the lipid deposition in livers, on preventing diabetes in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, and explore the probability of new mechanism in preventing diabetes in laboratory animals. Methods Twenty male spontaneous diabetic OLETF rats were treated as OLETF (group OLETF). Ten male Long-Evans Tokushima Otsuka (LETO) rats were used as normal control group(group LETO). Blood glucose is determined by oral glucose tolerance test. Rats were sacrificed at 8, 32 and 40 weeks and liver tissue isolated. Serum triglyceride, serum cholesterol, and serum free fatty acid (FFA) were determined. The morphological changes of livers were measured by HE stain. Based on HE results, frozen liver sections of OLETF rats of 32-week-old and 40-week-old respectively were stained with fatty Sudan Ⅲ as well as PAS before and after amylase digestion, the purpose of which is to further clarify the nature of the lesions. Results OLETF rats developed diabetes since 23 weeks of age and the incidence rate of diabetes became higher with the time goes by until got to 92.9% at 40 weeks of age when the research came to the end. No rats have developed diabetes or IGT in group LETO. The serum triglyceride, serum cholesterol, serum FFA was significantly lower in group LETO than that of group OLETF since 32 weeks (P < 0.05). With the week age increasing, liver vacuolation in group OLETF became serious and it was suggested that there appeared a gradual increase on liver fat infiltration and glycogen accumulation with a further improvement on the specific staining of fat and glycogen; while there was no significant damage in the liver structure of group LETO. Conclusion Hepatic insulin resistance may play an important part in the onset of T2DM, and the improvement of hepatic insulin resistance may work as a probable mechanism of preventing diabetes.
李佳1 薛耀明2 潘永华2 朱波2 张燕2. 肝脏胰岛素抵抗在自发性糖尿病OLETF大鼠发病中的作用[J]. 中国医药导报, 2018, 15(14): 12-16.
LI Jia1 XUE Yaoming2 PAN Yonghua2 ZHU Bo2 ZHANG Yan2. The role of Hepatic insulin resistance in the pathogenesis of spontaneously diabetic OLETF rats. 中国医药导报, 2018, 15(14): 12-16.
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2018, Vol. 15 
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