The microscopical indentification and determination of the content of five main components of Tagetes erecta L.
LYU Shaowa1 JIAN Jiping1 TAN Meiwei2 LIU Ying2 LYU Xinyu2 GUO Yuyan1 LI Guoyu2
1.Key Laboratory of Base and Application of Bei Chinese Materia Media, Ministry of Education, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.School of Pharmacy, Harbin University of Commerce, Heilongjiang Province, Harbin 150076, China
Abstract：Objective To provide theoretical basis for quality control and rational and effective development and utilization of Tagetes erecta L. by studying the microscopical characteristics of root, stem and leaf of marigold by means of microscopic identification, and detecting the contents of main components in different parts. Methods The microstructure features of Tagetes erecta L. were observed by microscopic identification. YMC C18 column (4.6 mm × 250 mm, 5 μm) was used as the column, acetonitrile (B) - 0.1% formic acid aqueous solution (A) was used as mobile phase of gradient elution with the detection wavelength of 257 nm and the colomn temperature was room temperature. The flow rate was 1.0 mL/min. The injection volume of the sample was 10 μL. Results 5,7,3′-trihydroxyl-3,6,4′-trimethoxylflavone (1), syringic acid (2), patulitrin (3), 5,3′-dihydroxyl-3,6,4′-trimethoxylflavone-7-O-β-D-glucopyranoside (4), 5-hydroxymethylfurfuryl methyl succinate (5) five kinds of linear regression equations were: Y = 2.7957X-0.1385, Y = 2.7951X-0.1163, Y = 0.1740X-0.0231, Y = 0.9006X-0.1242, Y = 0.1815X-0.0916. The correlation coefficient of the compounds was 0.099 92-0.099 95, the recovery rate of the sample was 96.43%-101.17%, and the RSD was 0.13%-1.75%. These five chemical components were found in the rhizome of Tagetes erecta L., and the contents of the other four compounds in the stem were larger than those in the root except for the compound 5. The content of compound 5 was the highest in the stem of Tagetes erecta L., up to 2.5%. Conclusion In this experiment, the effective microscopic identification of the roots, stems and leaves of marigold and the determination of five important components are carried out for the first time. The method is simple, accurate and reproducible, and provides a theoretical basis for the quality control of Tagetes erecta L..
吕邵娃1 菅计苹1 谭美微2 刘莹2 吕鑫宇2 郭玉岩1 李国玉2. 万寿菊的显微鉴别及5个主要成分的含量测定[J]. 中国医药导报, 2020, 17(4): 4-7.
LYU Shaowa1 JIAN Jiping1 TAN Meiwei2 LIU Ying2 LYU Xinyu2 GUO Yuyan1 LI Guoyu2. The microscopical indentification and determination of the content of five main components of Tagetes erecta L.. 中国医药导报, 2020, 17(4): 4-7.
2020, Vol. 17 
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