Analysis of UBQLN-1 expression and clinical significance in rectal cancer tissues and correlation with prognosis
SUN Guangyuan1 WU Xueliang1 XUE Jun1 ZHANG Haiyan2 MA Haiyan1 LIU Xiaoning3 HAN Lei1 QU Ming1
1.Department of General Surgery, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China;
2.Department of Neurology, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China;
3.Intensive Care Unit, 81th Army Hospital of the Army, Hebei Province, Zhangjiakou 075000, China
Abstract：Objective To explore the expression and clinical significance of ubiquitin-1 (UBQLN-1) in rectal cancer, and correlation analysis with prognosis . Methods From December 2015 to January 2016, a total of 90 patients who underwent rectal cancer surgery in the Department of General Surgery of the First Affiliated Hospital Hebei North University were selected. The rectal cancer foci and normal tissues adjacent to the cancer were collected. Reverse transcription polymerase chain reaction (Rt-PCR) was used. The expression of UBQLN-1 mRNA of specimens in the two groups were detected, while immunohistochemistry was used to detect the expression of UBQLN-1 protein of specimens in the two groups, and the relationship with the clinicopathological characteristics of the tumor was analyzed. The survival period of the included patients was followed up, Kaplan-Meier was used to draw survival curves, and the relationship between the expression of UBQLN-1 protein and the prognosis of rectal cancer patients were analyzed by multi-factor prognosis. Results The relative expression of UBQLN-1 mRNA in rectal cancer tissues was significantly higher than that in normal tissues adjacent to cancer, and the difference was highly statistically significant (P < 0.01). The positive expression of UBQLN-1 in rectal cancer tissues was significantly higher than that in normal tissues adjacent to cancer, and the difference was highly statistically significant (P < 0.01). The positive expression of UBQLN-1 in rectal cancer tissues of patients with different levels of differentiation, T stage, lymph node metastasis and liver metastasis were compared, and the differences were statistically significant (P ＜ 0.05). The 5-year disease-free survival rate of rectal cancer patients with positive expression of UBQLN-1 was significantly lower than that of patients with negative expression, and the difference was statistically significant (P ＜ 0.05). T staging (HR = 1.246), lymph node metastasis (HR = 2.538), liver metastasis (HR = 2.256) and UBQLN-1 expression (HR = 5.820) were independent risk factors that affected the prognosis of patients (P ＜ 0.05). Conclusion The high expression of UBQLN-1 in rectal cancer tissue is closely related to the occurrence, development, invasion and metastasis of rectal cancer. It is also an independent risk factor for the prognosis of rectal cancer patients. Therefore, it can be used as a potential molecular biological indicator for early diagnosis and prognosis evaluation of rectal cancer.
孙光源1 武雪亮1 薛 军1 张海燕2 马海燕1 刘晓宁3 韩磊1 屈明1. UBQLN-1在直肠癌组织中的表达、临床意义及与预后的相关性分析[J]. 中国医药导报, 2020, 17(25): 33-37.
SUN Guangyuan1 WU Xueliang1 XUE Jun1 ZHANG Haiyan2 MA Haiyan1 LIU Xiaoning3 HAN Lei1 QU Ming1. Analysis of UBQLN-1 expression and clinical significance in rectal cancer tissues and correlation with prognosis. 中国医药导报, 2020, 17(25): 33-37.
[1] Bray F，Ferlay J，Soerjomataram I，et al. Global cancer statistics 2018：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin，2018，68（6）：394-424.
[2] 袁平，马杏凡.中国结直肠癌筛查效果meta分析[J].中国医药导报，2020，17（8）：106-111.
[3] 付极，林国乐.分子诊断技术在结直肠癌个体化精准治疗中的应用[J].中华胃肠外科杂志，2016，19（1）：22-26.
[4] 段佳文，武雪亮，牛玉春，等.西妥昔单抗和贝伐单抗在晚期结直肠癌一二线治疗中的研究进展[J].中国医药导报，2017，14（24）：50-53.
[5] Voegeli M，Wicki A. Neoadjuvant，adjuvant and palliative systemic therapy of colorectal cancer [J]. Ther Umsch，2018，75（10）：622-626.
[6] Kurlawala Z，Shah PP，Shah C，et al. The STI and UBA Domains of UBQLN1 Are Critical Determinants of Substrate Interaction and Proteostasis [J]. J Cell Biochem，2017，118（8）：2261-2270.
[7] Li X，Zhou J，Chen H，et al. The association between the UBQLN1 polymorphism and Alzheimer′s disease risk：A systematic review [J]. Cell Mol Biol（Noisy-le-grand），2017，63（5）：94-96.
[8] Yue Z，Wang S，Yan W，et al. Association of UBQ-8i polymorphism with Alzheimer′s disease in Caucasians：a meta-analysis [J]. Int J Neurosci，2015，125（6）：395-401.
[9] Luo L，Liu Y，Tu X，et al. Decreased expression of ubiquilin-1 following neonatal hypoxia-ischemic brain injury in mice [J]. Mol Med Rep，2019，19（6）：4597-4602.
[10] Kurlawala Z，Dunaway R，Shah PP，et al. Regulation of insulin-like growth factor receptors by Ubiquilin1 [J]. Biochem J，2017，474（24）：4105-4118.
[11] Brahim EB，Ayari I，Jouini R，et al. Expression of epidermal growth factor receptor （EGFR） in colorectal cancer：An immunohistochemical study [J]. Arab J Gastroenterol，2018，19（3）：121-124.
[12] Ghaedamini S，Nikbakht M，Soleimani M. Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors [J]. Middle East J Dig Dis，2020，12（1）：39-44.
[13] Adegoke OO，Qiao F，Liu Y，et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer′s Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-β Accumulation in Mice [J]. J Alzheimers Dis，2017，59（2）：575-590.
[14] Fang X，Trexler C，Chen J，et al. Ushering in the cardiac role of Ubiquilin1 [J]. J Clin Invest，2018，128（12）：5195-5197.
[15] Chen X，Ebelle DL，Wright BJ，et al. Structure of hRpn10 Bound to UBQLN2 UBL Illustrates Basis for Complementarity between Shuttle Factors and Substrates at the Proteasome [J]. J Mol Biol，2019，431（5）：939-955.
[16] Kim H，Goldberg A. UBL domain of Usp14 and other proteins stimulates proteasome activities and protein degradation in cells [J]. Proc Natl Acad Sci USA，2018，115（50）：E11642-E11650.
[17] Liu Y，Qiao F，Wang H，et al. Enhanced Proteostasis in Post-ischemic Stroke Mouse Brains by Ubiquilin-1 Promotes Functional Recovery [J]. Cell Mol Neurobiol，2017， 37（7）：1325-1329.
[18] Mizukami K，Abrahamson E，Mi Z，et al. Immunohistochemical analysis of ubiquilin-1 in the human hippocampus：association with neurofibrillary tangle pathology [J]. Neuropathology，2014，34（1）：11-18.
[19] Natunen T，Takalo M，Kemppainen S，et al. Relationship between ubiquilin-1 and BACE1 in human Alzheimer′s disease and APdE9 transgenic mouse brain and cell-based models [J]. Neurobiol Dis，2015，85（11）：187-205.
[20] Liu Y，Lü L，Hettinger CL，et al. Ubiquilin-1 protects cells from oxidative stress and ischemic stroke caused tissue injury in mice [J]. J Neurosci，2014，34（8）：2813-2121.
[21] Yadav S，Singh N，Shah PP，et al. MIR155 Regulation of Ubiquilin1 and Ubiquilin2：Implications in Cellular Protection and Tumorigenesis [J]. Neoplasia，2017，19（4）：321-332.
[22] Bao J，Jiang X，Zhu X，et al. Clinical significance of ubiquilin-1 in gastric cancer [J]. Medicine（Baltimore），2018， 97（3）：e9701.
[23] 付慧，李艳萍，汪云超，等.Ubiquilin1蛋白在乳腺癌组织中的表达与预后意义[J].肿瘤防治研究，2017，44（8）：535-539.
[24] 王静，贺英，王萍，等.卵巢癌组织中泛素1的表达变化及其临床意义[J].山东医药，2018，58（35）：61-63.
[25] 胡潺潺，李青山，刘兰芳，等.RNA干扰UBQLN-1基因表达对乳腺癌细胞增殖、凋亡、侵袭的影响及机制研究[J].中国免疫学杂志，2019，34（9）：1315-1320.
2020, Vol. 17 
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