Effect study on MTDH mediated baicalin inhibiting proliferation, migration and invasion of 4T1 breast cancer cells
ZHANG Hui1 HUANG Lizhong2 XIAO YuJie2 DENG Tianhao3 GONG Hui3
1.Department of Medical Oncology, Chinese Medicine Hospital of Hainan Province, Hainan Province, Haikou 570203, China;
2.College of Combination of Chinese and Western Medicine, Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410007, China;
3.Department of Medical Oncology, Hu′nan Academy of Traditional Chinese Medicine Affiliated Hospital, Hu′nan Province, Changsha 410006, China
Abstract：Objective To investigate the effects of baicalin on proliferation, migration and invasion of 4T1 cells based on metadherin (MTDH) gene. Methods 4T1 cells in logarithmic growth stage were divided into blank control group and baicalin group with different doses of 25, 50, 100, 150 mg/L and 200 mg/L, and then treated with corresponding drugs. Cell activity after baicalin treatment was detected by MTT method. Migration was measured by scratch assay. Invasion was measured by transwell assay and MTDH expression in cells was measured by Western blot. Results The effect of baicalin at 25 mg/L on cell migration was not obvious. As the concentration increased to 50 mg/L, the inhibition of cell migration gradually became obvious. When the concentration increased to 100 mg/L and 150 mg/L, the inhibition of cell migration showed obvious effects. The inhibition rate of cell invasion of baicalin groups with different concentrations was statistically significant (P ＜ 0.05). The inhibition rate of cell invasion in the group of 150 mg/L baicalin was higher than that in the group of 100 mg/L baicalin (P ＜ 0.05). The inhibition rate of cell invasion in the 100 mg/L and 150 mg/L baicalin group was higher than that in the 50 mg/L baicalin group, and the differences were highly statistically significant (P ＜ 0.01). Compared with the control group, MTDH expression was decreased in the 50, 100 mg/L and 150 mg/L baicalin groups (P ＜ 0.05 or P ＜ 0.01). Conclusion Baicalin can inhibit the growth and proliferation of 4T1 breast cancer cells, inhibit the migration and invasion ability of breast cancer cells, and its mechanism may be related to the down-regulation of MTDH expression.
张慧1 黄立中2 肖玉洁2 邓天好3 龚辉3. MTDH介导黄芩苷抑制4T1乳腺癌细胞增殖、迁移和侵袭的作用研究[J]. 中国医药导报, 2020, 17(24): 16-19,27.
ZHANG Hui1 HUANG Lizhong2 XIAO YuJie2 DENG Tianhao3 GONG Hui3. Effect study on MTDH mediated baicalin inhibiting proliferation, migration and invasion of 4T1 breast cancer cells. 中国医药导报, 2020, 17(24): 16-19,27.
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