LI Xiaojing1 WEI Yingjie1,2 WANG Jing1,2 XU Huan1 HUANG Yiping1,2 Janagul·As3 CHEN Bin1,2
1.The Third Clinical Medical College, Nanjing University of Chinese Medicine, Jiangsu Province, Nanjing 210028, China;
2.Key Laboratory for Traditional Chinese Medicine Drug Release System Under State Administration of Traditional Chinese Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Jiangsu Province, Nanjing 210028, China;
3.Xinjiang Yili Kazak Autonomous Prefecture Hospital of Traditional Chinese Medicine, Xinjiang Uygur Autonomous Region, Yining 835000, China
Abstract：Objective To establish a quality control method for Ma′nao Gao. Methods Thin layer chromatography was applied to the qualitative identification of Faeces Togopteri, Corydalis Rhizoma, Rhei Radix et Rhizoma, Psoraleae Fructus, Notoginseng Radix et Rhizoma. High performance liquid chromatography was used for the content determination of tetrahydropalmatine, acetonitrile-0.1% triethylamine phosphate system was used as the mobile phase of gradient elute. The column temperature was 30°C and the detection wavelength was set at 280 nm. Results Thin layer chromatography spots was clear, which had good resolution without interference. Tetrahydropalmatine had good linear relationship with the range of 2.022-20.22 μg/mL (r = 0.9999) respectively, the average recovery was 100.27% (RSD = 2.39%). Conclusion The method is reliable with good stability and good repeatability, and can be used for quality control of Ma′nao Gao.
2020, Vol. 17 
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