Abstract：Objective To explore the relationship between specific allergens and T cell activation during anaphylactic shock caused by cystic echinococcosis, and to provide new ideas for clinical treatment. Methods Serum was collected from 30 patients (research group) who were underwent cystic hydatid cystectomy in the First Affiliated Hospital of Xinjiang Medical University from January 2015 to December 2017. The patients with anaphylactic shock during the operation were taken as the anaphylactic shock group (10 cases), while patients without anaphylactic shock were taken as the non-anaphylactic shock group (20 cases), and the blood samples of healthy examinees from the same period were selected as the control group (30 people). The levels of serum immunoglobulin (Ig) E and IgG1 in each group were analyzed, while the expression levels of interleukin (IL)-10, IL-4, IL-2 and γ interferon (IFN-γ) in the serum of each group were detected, and correlation analysis was performed. Results The expression levels of IgE and IgG1 during the operation in the anaphylactic shock group were higher than those in the non-anaphylactic shock group, and the differences were statistically significant (P ＜ 0.05). There were no statistically significant differences in serum IgE and IgG1 expression levels among the groups after 1 week of operation (P ＞ 0.05). The expression levels of serum IL-4 and IL-10 before the operation in the study group were higher than those in the control group, and the differences were statistically significant (P ＜ 0.05). The serum IL-4 and IL-10 during the operation in the anaphylactic shock group were lower than those in the non-anaphylactic shock group, while the IFN-γ and IL-2 were higher than those in the non-anaphylactic shock group, and the differences were statistically significant (P ＜ 0.05). The serum IL-4 and IL-10 expression levels of the anaphylactic shock group were higher than those of the non-anaphylactic shock group and the control group after 1 week of operation, and the differences were statistically significant (P ＜ 0.05). IgE was positively correlated with IL-2 and IFN-γ (r = 0.96, 0.68, P ＜ 0.05), while IgG1 was positively correlated with IL-2 and IFN-γ (r = 0.60, 0.79, P ＜ 0.05). Conclusion As long as anaphylactic shock occurs, Th1 and Th2 type cells are imbalanced. Th1 type plays a leading role in anaphylactic shock, and its activation is related to IgE and IgG1.
李杰 戴庆 亚力·亚森 钮月 马岩. 囊性包虫病所致过敏性休克中IgE、IgG1与T细胞激活的关系[J]. 中国医药导报, 2020, 17(19): 93-96.
LI Jie DAI Qing Yali·Yasen NIU Yue MA Yan. Relationship between the expression of IgE, IgG1 and T cell activation in anaphylactic shock caused by cystic echinococcosis. 中国医药导报, 2020, 17(19): 93-96.
[1] 李亦梅.包虫病所致过敏性休克患者免疫学保护性因素的研究[D].乌鲁木齐：新疆医科大学，2011.
[2] Thompson RC. The taxonomy，phylogeny and transmission of Echinococcus. Exp Parasitol [J]. Exp Parasitol，2008， 119（4）：439-446.
[3] Moro P，Schantz PM. Echinococcosis：a review [J]. Int J Infect Dis，2009，13（2）：125-133.
[4] Nunnari G，Pinzone MR，Gruttadauria S，et al. Hepatic ech-inococcosis：clinical and therapeutic aspects [J]. World Gastroenterol，2012，18（13）：1448-1458.
[5] Adel-Patient K，Bernard H，Ah-Leung S，et al. Peanut- and cow′s milk-specific IgE，Th2 cells and local anaphylactic reaction are induced in Balb/c mice orally sensitized with cholera toxin [J]. Allergy，2015，60（5）：658-664.
[6] Li S，Qian R，Wang S，et al. Identification of IgE and IgG1 specific antigens in Echinococcus granulosus cyst fluid [J]. Braz J Med Biol Res，2017，50（7）：e6071.
[7] 郑宏，徐志新，杨戈雄，等.感染细粒棘球蚴绵羊诱发过敏性休克期间IgG、IgG1、IgE抗体水平的探讨[J].中国寄生虫学与寄生虫病杂志，2003，21：42-45.
[8] Zhou X，Wang W，Cui F，et al. Extracellular vesicles derived from Echinococcus granulosus hydatid cyst fluid from patients： isolation，characterization and evaluation of immunomodulatory functions on T cells [J]. Int J Parasitol，2019，49（13/14）：1029-1037.
[9] Yue X，Li H，Tang J，et al. Rapid and label-free screening of echinococcosis serum profiles through surface-enhanced Raman spectroscopy [J]. Anal Bioanal Chem，2019， 25（3）：161-168.
[10] Moghaddam SM，Picot S，Ahmadpour E. Interactions between hydatid cyst and regulated cell death may provide new therapeutic opportunities [J]. Parasite，2019，26（1）：70.
[11] Bayraktar MR，Mehmet N，Durmaz R. Th1 and Th2 inducing cytokines in Cystic echinococcosis [J]. Turkiye Parazitol Derg，2005，29（3）：167-170.
[12] SamPson HA，Munoz-Furlong A，Bock SA，et al. SymPosium on the definition and management of anaphylaxis：Summary report [J]. J Allergy Clin Immunol，2005，115（3）：584-591.
[13] 谷美龄，李亦梅，郑宏.囊型包虫病致过敏性休克患者IFN-γ和IL-4的表达及其意义[J].中国病原生物学杂志，2011，6（3）：34-38.
[14] Li Y，Zheng H，Gu M，et al. Comparisons of serum total IgE，IgG，and IgG1 levels in patients with and without echinococcosis-induced anaphylactic shock [J]. Am J Trop Med Hyg，2012，87（1）：104-108.
[15] Siracusano A，Margutti P，Delunardo F，et al. Molecular cross-talk in host-parasite relationships：he intriguing immunomodulatory role of Echinococcus antigen B in cystic echinococcosis [J]. Int J Parasitol，2008，38（12）：1371-1376.
[16] Almond RJ，Flanagan BF，Antonopoulos A，et al. Differential immunogenicity and allergenicity of native and recombinant human lactoferrins：Role of glycosylation [J]. Eur J Immunol，2013，43（1）：170-181.
[17] Zhang Q，Ye J，Zheng H. Dexamethasone attenuates ech-inococcosis-induced allergic reactions via regulatory T cells in mice [J]. BMC Immunology，2016，17（1）：4.
[18] Furuhashi K，Chua YL，Wong KHS，et al. Priming with high and low respiratory allergen dose induces differential CD4+ T helper type 2 cells and IgE/IgG1 antibody responses in mice [J]. Immunology，2017，151（2）：227.
[19] Turquetineves A，Otte M，Schwartz C，et al. The Extracellular Domains of IgG1 and T Cell-Derived IL-4/IL-13 Are Critical for the Polyclonal Memory IgE Response In Vivo [J]. PLoS Biol，2015，13（11）：112-119.
[20] Dematteis S，Baz A，Rottenberg M，et al. Antibody and Th1/Th2-type responses in BALB/c mice inoculated with live or dead Echinococcus granulosus protoscoleces [J]. J Helminthol，2010，21（1）：19-26.
2020, Vol. 17 
京公网安备 11010502046598号