Abstract：Objective To investigate the effects of cryoprotective agents with different proportions on transplantation of frozen human granular fat in nude mice in order to optimize the selection of cryoprotectants. Methods The excess granular fat after liposuction on the inner side of the thigh was taken into the cryoprotective agents of A and B in different proportions, group A and group B respectively and they were stored at -80°C for 6 months. After 6 months, the specimens were resuscitated and transplanted subcutaneously into the back of nude mice, and 0.2 mL granular fat was injected at each transplant site. After 4 weeks, the nude mice were sacrificed, and the grafts were taken out for volume and weight detection, and observed under hematoxylin-eosin (HE) staining and transmission electron microscopy. Results The mean volume of residual fat in group A was greater than that in group B, and the difference was statistically significant (P < 0.05). HE staining showed that the neovascularization of group A was more than that of group B. Under transmission electron microscope, the fat cells of group A were more complete and there were more new fat cells. Conclusion The long-term frozen fat of group A cryoprotective agents has higher activity, and fetal bovine serum can be replaced by calf serum. The adipose tissue can still be effectively transplanted after cryopreservation for half a year.
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2019, Vol. 16 
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