Changes of C-type natriuretic peptide expression in different tissues of mice with chronic stress-induced depression
REN Jingyi1 FU Yuanyuan1 JING Xiaotong2 SU Yujiao2 SHI Xiaorui2 DU Yuru2 SHI Haishui2
1.School of Public Health, Hebei Medical University, Hebei Province, Shijiazhuang 050017, China;
2.Center of Neuroscience Research, Institute of Medical and Health Science, Hebei Medical University, Hebei Province, Shijiazhuang 050017, China
Abstract：Objective To investigate the effects of C-type natriuretic peptide (CNP) expression in different tissues and serum of mice with chronic stress-induced depression. Methods Twenty male ICR mice were selected and divided into control group and stress group according to the random number table method with 10 mice in each group. Chronic mild stress (CMS) method was used to establish depression mice model. After animal behavior test, samples were taken and the levels of CNP in hippocampus, liver, kidney, heart and serum were quantitatively analyzed by enzyme-linked immunosorbent assay (ELISA). Results The results of behavioral test showed that compared with the control group, the preference value of sugar and water in stress group was significantly reduced, the immobility latency of forced swimming test and tail suspension test were significantly shortened, and the immobility time of forced swimming was significantly prolonged, the differences were statistically significant (P < 0.05). There was no significant difference in total water intake between the two groups (P > 0.05). Compared with the control group, the levels of CNP in the heart, kidney and serum of the stress group increased significantly (all P < 0.05). Conclusion Chronic stress can significantly increase the levels of CNP in heart, kidney and serum, suggesting that the changes of CNP expression in peripheral tissues may be related to the occurrence and development of stress-induced depression.
任敬一1 付媛媛1 荆潇橦2 苏玉娇2 史晓瑞2 杜玉茹2 史海水2. 慢性应激致抑郁小鼠不同组织C型钠尿肽表达的变化[J]. 中国医药导报, 2019, 16(22): 26-29.
REN Jingyi1 FU Yuanyuan1 JING Xiaotong2 SU Yujiao2 SHI Xiaorui2 DU Yuru2 SHI Haishui2. Changes of C-type natriuretic peptide expression in different tissues of mice with chronic stress-induced depression. 中国医药导报, 2019, 16(22): 26-29.
[1] Dyussenova L，Pivina L，Semenova Y，et al. Associations between depression，anxiety and medication adherence among patients with arterial hypertension：Comparison between persons exposed and non-exposed to radiation from the Semipalatinsk Nuclear Test Site [J]. J Environ Radioact，2018，195：33-39.
[2] 方飞.舍曲林联合心理疗法治疗46例冠心病合并抑郁症患者的临床研究[J].中华全科医学，2017，15（7）：1204-1206.
[3] 郑宋宋，徐亚青，周玉琴，等.糖尿病肾病合并抑郁症危险因素分析[J].浙江中西医结合杂志，2018，28（10）：878-880.
[4] Patel R，Shristi S，Hina S. Comorbidities and Consequences in Hospitalized Heart Failure Patients with Depression [J]. Cureus，2018，10（8）：e3193.
[5] 刘鹏，姚睿智，刘洋，等.冷应激对大鼠外周组织中葡萄糖代谢的影响[C]//中国生理学会内分泌代谢、比较生理与应激生理学术会议，2017.
[6] Piantino J，Goldenberg F，Pytel P，et al. Progressive intracranial fusiform aneurysms and T-cell immunodeficiency [J]. Pediatr Neurol，2013，48（2）：130-134.
[7] Serra R，Buffone G，Falcone D，et al. Chronic venous leg ulcers are associated with high levels of metalloproteinases-9 and neutrophil gelatinase-associated lipocalin [J]. Wound Repair Regen，2013，21（3）：395-401.
[8] 牛雅娟.《中国抑郁障碍防治指南》药物治疗解读[J].临床药物治疗杂志，2018，16（5）：6-8.
[9] 顾思梦，李洋，杜文东.《内经》理论指导抑郁症从肝辨治的探讨[J].时珍国医国药，2014，25（2）：420-421.
[10] Liu X，Shi S，Yang H，et al. The activation of N-methyl-d-aspartate receptors downregulates transient outward potassium and L-type calcium currents in rat models of depression [J]. Am J Physiol Cell Physiol，2017，313（2）：C187-C196.
[11] 胡智玲，杨爽，杜玉茹，等.应激致抑郁行为小鼠血清TPO和ANG的变化[J].河北医科大学学报，2018，39（8）：887-889.
[12] Yau S，Li A，Hoo R，et al. Physical exercise-induced hippocampal neurogenesis and antidepressant effects are mediated by the adipocyte hormone adiponectin [J]. Proc Natl Acad Sci U S A，2014，111（44）：15 810-15 815.
[13] Karnib N，El-Ghandour R，El HL，et al. Lactate is an antidepressant that mediates resilience to stress by modulating the hippocampal levels and activity of histone deacetylases [J]. Neuropsychopharmacology，2019，44（6）：1152-1162.
[14] Kellner M，Wiedemann K，Holsboer F. Atrial natriuretic factor inhibits the CRH-stimulated secretion of ACTH and cortisol in man [J]. Life Sci，1992，50（24）：1835-1842.
[15] He W，Zhou Q，Yu S，et al. Mechanism on atrial natriuretic peptide receptor in anti-anxiety with acupuncture based on its tranquilizing effect [J]. Zhongguo Zhen Jiu，2015，35（1）：101-104.
[16] Kaczmarczyk M，Otte C，Wiedemann K，et al. Major depression and atrial natriuretic peptide：The role of adverse childhood experiences [J]. Psychoneuroendocrinology，2018，101：7-11.
[17] Strohle A，Jahn H，Montkowski A，et al. Central and peripheral administration of atriopeptin is anxiolytic in rats [J]. Neuroendocrinology，1997，65（3）：210-215.
[18] Fangauf S，Herbeck B，Meyer T，et al. Associations of NT-proBNP and parameters of mental health in depressed coronary artery disease patients [J]. Psychoneuroendocrinology，2018，96：188-194.
[19] 陆矫.C型利钠肽及受体在运动预适应诱导心肌保护效应中的变化及机制[D].上海：上海体育学院，2015.
[20] Wu L，Zhang Q，Zhang S，et al. Effects of gene knockdown of CNP on ventricular remodeling after myocardial ischemia-reperfusion injury through NPRB/Cgmp signaling pathway in rats [J]. J Cell Biochem，2018，119（2）：1804-1818.
[21] Wang L，Liu W，Yu Y，et al. Increased circulating bioactive C-type natriuretic peptide is associated with reduced heart rate variability in patients with chronic kidney disease [J]. BMC Nephrol，2018，19（1）：50.
[22] Bae C，Hino J，Hosoda H，et al. C-type natriuretic peptide （CNP） in endothelial cells attenuates hepatic fibrosis and inflammation in non-alcoholic steatohepatitis [J]. Life Sci，2018，209：349-356.
[23] Decker J，Wojtowicz A，Heinemann U，et al. C-Type natriuretic peptide modulates pre- and postsynaptic properties in hippocampal area CA1 in vitro[J]. Biochem Biophys Res Commun，2008，377（3）：820-825.
[24] Decker J，Wojtowicz A，Ul H，et al. C-type natriuretic peptide decreases hippocampal network oscillations in adult rats in vitro [J]. Neuroscience，2009，164（4）：1764-1775.
[25] Lin X，Tang X，Cai Z，et al. NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF [J]. PLoS One，2016，11（2）：e149031.
2019, Vol. 16 
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