蜗牛匀浆液直肠给药对溃疡性结肠炎大鼠的保护作用

摘要
图/表
参考文献(20)
相关文章 (11)

全文: PDF (705 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨蜗牛匀浆液直肠给药对溃疡性结肠炎大鼠是否具有保护作用。方法用冰醋酸诱导急性溃疡性结肠炎大鼠模型，将40只6～7周龄的SPF级雄性SD大鼠采用随机数字表法分为模型组、空白组、蜗牛匀浆液的高剂量组（1.0 g/kg）和低剂量组（0.5 g/kg），每组各10只。造模后，高、低剂量组连续直肠给予蜗牛匀浆液7 d，空白组与模型组均给予相同体积的生理盐水。每日观察大鼠的主要体征与粪便性状，同时计算体重变化并进行粪便评分。实验结束后，处死大鼠取目标结肠段，称量计算肠重系数，同时采用HE染色法进行病理组织检测。结果与模型组比较，高剂量组和低剂量组大鼠体征明显改善，大鼠体重增加程度更显著（P ＜ 0.05或P ＜ 0.01），并且肠重系数显著降低（P ＜ 0.01），高剂量组给药后第4天开始出现粪便评分显著下降（P ＜ 0.05或P ＜ 0.01）。同时，高剂量组炎性细胞浸润及炎症细胞浸润深度评分和溃疡深度评分与模型组比较，差异有高度统计学意义（P ＜ 0.01）。结论蜗牛匀浆液对溃疡性结肠炎模型大鼠可通过改善体征、增加体重、降低腹泻、对抗结肠组织水肿及炎性反应等产生较好的保护作用，尤以高剂量组作用明显。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	范志源余秋雨李欢苏小娇盛艳梅

关键词 ：蜗牛匀浆液, 溃疡性结肠炎, 直肠给药, 保护作用

Abstract：Objective To investigate whether snail homogenate has protective effect on ulcerative colitis in rats. Methods Rat model of acute Ulcerative Colitis induced by glacial acetic acid. Forty male Sprague-Dawley rats aged 6-7 weeks were randomly divided into model group, blank group, snail homogenate high (1.0 g/kg) and low (0.5 g/kg) dose groups according to random number table, with 10 rats in each group. After the model was completed, the high (1.0 g/kg) and low (0.5 g/kg) dose groups were given snail homogenate continuously for 7 days in the rectum, and the blank group and the model group were given the same volume of normal saline. The main signs and fecal traits of the rats were observed daily, and body weight changes were calculated and fecal scores were performed. At the end of the experiment, the rats were sacrificed and the target colon segment was taken. The coefficient of intestinal weight was calculated and the pathological tissue was detected by HE staining. Results Compared with model group, the symptoms in rats of high-dose group and low-dose group were significantly improved, the degree of weight gain of rats increased significantly (P ＜ 0.05 or P ＜ 0.01), and intestinal weighting coefficient reduced significantly (P ＜ 0.05 or P ＜ 0.01), a significant decrease in fecal scores of high-dose group began on day 4 after administration (P ＜ 0.01). Meanwhile, the scores of inflammatory cell infiltration depth and ulcer depth in the high-dose group were significantly different from those in the model group (P ＜ 0.01). Conclusion Snail homogenate can improve the physical signs, increase body weight, reduce diarrhea, and fight colonic hyperplasia and inflammatory reaction in rats with ulcerative colitis, especially at high doses.

Key words： Snail homogenate Ulcerative colitis Rectal administration Protective effect

基金资助:四川省大学生创新创业项目（201613705021）。

通讯作者: 盛艳梅（1979-），女，博士，教授；研究方向：中药药效与毒理学。

作者简介: 范志源（1995-），男，成都医学院药学院2018级药品研究与注册专业在读硕士研究生；研究方向：中药药效与毒理学。

引用本文:

范志源余秋雨李欢苏小娇盛艳梅. 蜗牛匀浆液直肠给药对溃疡性结肠炎大鼠的保护作用[J]. 中国医药导报, 2019, 16(22): 17-20,25.
FAN Zhiyuan YU Qiuyu LI Huan SU Xiaojiao SHENG Yanmei. Protective effect of snail homogenate enema on ulcerative colitis in rats. 中国医药导报, 2019, 16(22): 17-20,25.

链接本文:

http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2019/V16/I22/17

[1] 王丽梅，相祎，吴颖，等.甘露聚糖肽联合美沙拉嗪治疗溃疡性结肠炎的临床效果[J].中国医药导报，2018，15（33）：89-92.
[2] 秦菲，叶柏.中西医结合治疗溃疡性结肠炎的研究进展[J].中医药导报，2015，21（14）：99-101.
[3] 胡倩，罗怀岗，李五生.中药灌肠对溃疡性结肠炎的治疗进展[J].临床合理用药杂志，2016，9（4）：167-168.
[4] 李晓玲.美沙拉秦不同给药途径治疗活动期溃疡性结肠炎的疗效及对血清炎性因子的影响[J].河北医学，2016， 22（10）：1595-1597.
[5] 南京中医药大学.中药大辞典[M].2版.上海：上海科学技术出版社，2006：3477-3479.
[6] Bonnemain B. Helix and Drugs：Snails for Western Health Care From Antiquity to the Present [J]. Evid Based Complement Alterna Med，2005，2（1）：25-28.
[7] Tsoutsos D，Kakagia D，Tamparopoulos K. The efficacy of Helix aspersa Müller extract in the healing of partial thickness burns：A novel treatment for open burn management protocols [J]. J Dermatological Treatment，2009，20（4）：219-222.
[8] Brieva A，Philips N，Tejedor R，et al. Molecular Basis for the Regenerative Properties of a Secretion of the Mollusk Cryptomphalus aspersa [J]. Skin Pharmacol Physiol，2008， 21（1）：15-22.
[9] Sallam AAA，El-massry SA，Nasr IN. Chemical analysis of mucus from certain land snails under Egyptian conditions [J]. Archives Phytopathol Plant Protect，2009，42（9）：874-881.
[10] Mubarak MASE，Lamari FN，Kontoyannis C. Simultaneous determination of allantoin and glycolic acid in snail mucus and cosmetic creams with high performance liquid chromatography and ultraviolet detection [J]. J Chromatography A，2013，1322（24）：49-53.
[11] Dimosthenis T，Despoina K，Konstantinos T. The efficacy of Helix aspersa Müller extract in the healing of partial thickness burns：a novel treatment for open burn management protocols [J]. J Dermatological Treatment，2009， 20（4）：219-222.
[12] Ziomek M，Drozd A，Cha?覥abis-mazurek A，et al. Concentration levels of cadmium and lead in the raw and processed meat of Helix pomatia snails [J]. Pol J Vet Sci，2018，21（3）：483-489.
[13] Pandey A，Verma S，Kumar VL. Metformin maintains mucosal integrity in experimental model of colitis by inhibiting oxidative stress and pro-inflammatory signaling [J]. Biomed Pharmacother，2017，94：1121-1128.
[14] 母小东，韩玉梅，邹丽.参肠片治疗溃疡性结肠炎的实验研究[J].西南军医，2009（2）：235-237.
[15] 陈怀涛.兽医病理学原色图谱[M].中国农业出版社，2008：694.
[16] Ulagesan S，Kim H. Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails [J]. Appl Sci，2018，8（8）：1362.
[17] Greistorfer S，Klepal W，Cyran N，et al. Snail mucus-glandular origin and composition in Helix pomatia [J]. Zoology，2017，122：126-138.
[18] Ellijimi C，Ben HM，Othman H，et al. Helix aspersa maxima mucus exhibits antimelanogenic and antitumoral effects against melanoma cells [J]. Biomed Pharmacother，2018，101：871-880.
[19] Trapella C，Rizzo R，Gallo S，et al. HelixComplex snail mucus exhibits pro-survival，proliferative and pro-migration effects on mammalian fibroblasts [J]. Sci Rep，2018，（8）：1-10.
[20] Bortolotti D，Trapella C，Bernardi T，et al. Letter to the Editor：Antimicrobial properties of mucus from the brown garden snail Helix aspersa [J]. Br J Biomed Sci，2016，73（1）：49-50.