地肤子总黄酮对二硝基氯苯致大鼠接触性皮炎的抗炎作用

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摘要目的研究地肤子总黄酮（FFK）对二硝基氯苯（DNBC）致大鼠变态反应性接触性皮炎（ACD）的抗炎作用。方法 SPF级SD大鼠40只，雌雄各半，按随机数字表法分为五组，每组8只，即空白对照组、ACD模型组、阳性药对照组（醋酸泼尼松龙2.5 mg/kg）、FFK低剂量组（75 mg/kg）、FFK高剂量组（150 mg/kg）；以DNBC建立大鼠ACD模型后，予以FFK灌胃给药，连续10 d。观察皮肤变态性反应和肿胀度，同时测定血清中白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和γ干扰素（IFN-γ）表达水平，并通过免疫组化检测耳组织中核因子κB p65（NF-κB p65）表达水平。结果与空白对照组比较，ACD模型组大鼠皮肤肿胀度明显增高，血清中IL-6、IFN-γ含量与耳组织中NF-κB p65蛋白表达明显增高，而血清中IL-10含量明显降低（P < 0.01）；与ACD模型组比较，FFK低剂量组与FFK高剂量组大鼠的耳肿胀度、血清中IL-6、IFN-γ含量、耳组织中NF-κB p65蛋白表达水平显著降低（P < 0.01），而血清中IL-10含量显著升高（P < 0.01）。结论 FFK对于DNBC致大鼠ACD具有抗炎作用，其机制可能是通过抑制NF-κB p65蛋白发挥作用。

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	蔡霈文晓柯欧阳波肖作奇

关键词 ：地肤子总黄酮, 炎症, 接触性皮炎, 核转录因子-&kappa, B p65

Abstract：Objective To investigate the anti-inflammatory effect of the total flavonoids of Fructus Kochiae (FFK) on DNBC-induced allergic contact dermatitis (ACD) rats. Methods According to random number table method, 40 half male and half female SPF grade SD rats were randomly divided into 5 groups, with 8 rats in each group, including blank control group, ACD model group, positive control group (Splash Methylprednisolone 2.5 mg/kg), FFK low-dose group (75 mg/kg), FFK high-dose group (150 mg/kg). After ACD model were established by treatment of DNCB on right ear for 10 days, rats were treated with FFK in 10 d and the allergic reaction and swelling degree of ears were observed, the content of IL-6, IL-10 and IFN-γ in serum and the level of NF-κB p65 in ears were determined. Results Compared with the blank control group, the swelling of ears in ACD model group was significantly increased, the content of IL-6, IFN-γ in serum and the level of NF-κB p65 in ears were up-regulated, while the content of IL-10 was down-regulated in serum (P < 0.01). Compared with the ACD model group, the ears swelling degree of rats in FFK low-dose group and FFK high-dose group was significantly decreased, the content of IL-6, IFN-γ in serum and the level of NF-κB p65 in ears were significantly down-regulated, while the content of IL-10 in serum was significantly up-regulated (P < 0.01). Conclusion FFK has anti-inflammatory effect on DNBC-induced ADC rats and its mechanism involved in down-regulate the NF-κB p65.

Key words： Total flavonoids of Fructus Kochiae Inflammation Allergic contact dermatitis NF-κB p65

基金资助:湖南省中医药科研计划项目（201873）。

通讯作者: 肖作奇（1987.12-），男，博士，主要从事中药物质基础及活性研究。

引用本文:

蔡霈文晓柯欧阳波肖作奇. 地肤子总黄酮对二硝基氯苯致大鼠接触性皮炎的抗炎作用[J]. 中国医药导报, 2019, 16(20): 17-20.
CAI Pei WEN Xiaoke OUYANG Bo XIAO Zuoqi. Anti-inflammatory effect of the total flavonoids of Fructus Kochiae in DNBC-induced allergic contact dermatitis rats. 中国医药导报, 2019, 16(20): 17-20.

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