Study on chemical constituents and antitumor activity of Gentiana triflora Pall.
ZHOU Yanli1 WANG Dali2 ZHANG Yan1 ZHANG Ning1 LI Shuoxi1 WU Jiao3 LI Yang3
1.Jiamusi College of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Jiamusi 154007, China;
2.Experimental Center of Dalian Children′s Hospital, Liaoning Province, Dalian 116012, China;
3.College of Life Science, Dalian Nationalities University, Liaoning Province, Dalian 116600, China
Abstract：Objective To investigate the chemical constituents and antitumor activities in vitro of Gentiana triflora Pall. Methods The root of Gentiana triflora Pall. was extracted with 95% ethyl alcohol, which is extracted with petroleum ether, ethyl acetate and n-butanol, successively. The extracts were separated and purified by silica gel column chromatography and preparative high performance liquid chromatography. The compounds were structurally identified according to physicochemical properties and spectral data. The antitumor activities of the compounds were evaluated by microculture tetrozolium (MTT) method. Results Five compounds were isolated from the ethyl acetate and n-butanol extraction sites and identified as globuloside A (1), cornusoside A (2), cornolactone A (3), 6, 9-epi-8-O-Acetylshanziside methyl ester ( 4), 5,9-epi-Penstemoside (5); anti-tumor activity experiments showed that Compound 1 had a certain inhibitory effect on the proliferation of HepG2 cells in vitro, with an IC50 value of 6.7 μmol/L. Conclusion All the compounds are isolated from Gentiana triflora Pall. firstly. Compound 1 has significant inhibitory activity on the proliferation of HepG2 cells.
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2019, Vol. 16 
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