负压封闭引流治疗C57BL/6J烧伤脓毒症小鼠的效果

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摘要目的探讨负压封闭引流（VSD）治疗C57BL/6J烧伤脓毒症小鼠的效果。方法选取雄性C57BL/6J小鼠300只，建立烧伤脓毒症小鼠模型；取造模成功的80只烧伤脓毒症小鼠按照随机数字表法将其分为实验组和对照组，每组各40只。实验组创面处理并采用VSD治疗，接负压治疗仪，调节负压为-120 mmHg（1 mmHg=0.133 kPa）；对照组仅创面处理，每隔24 h更换敷料一次。统计两组创面细菌荧光强度，创面皮肤血流量情况，白介素（IL）-1β、IL-1、IL-6、IL-8、IL-10、肿瘤坏死因子-α（TNF-α）等炎性细胞因子水平，观察7 d累计生存率。结果治疗前，两组的创面荧光强度相似，差异无统计学意义（P > 0.05）；与治疗前比较，治疗24 h后对照组荧光信号强度显著升高（P < 0.05），实验组荧光强度降低（P < 0.05），且两组比较，差异有统计学意义（P < 0.05）。与治疗前比较，对照组经治疗第1、2天有所降低，第3、5、7天后陆续升高，差异均有统计学意义（P < 0.05或P < 0.01）；实验组治疗第1、2、3、5、7天小鼠背部创面血流量逐渐增加且明显高于对照组，差异均有统计学意义（P < 0.05或P < 0.01）。经治疗，实验组促炎因子IL-1β、IL-6、TNF-α含量较对照组明显减少，IL-8含量较对照组明显增加，差异均有统计学意义（均P < 0.05），IL-10含量变化不明显（均P > 0.05）。从第2天开始，实验组生存率明显高于对照组，差异均有统计学意义（均P < 0.05）。结论 VSD治疗C57BL/6J烧伤脓毒症小鼠，可降低创面细菌含量，改善血液供应，降低脓毒症的全身炎性反应水平，提高生存率。

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	刘洋白晓智李小强陈子阅何亭▲

关键词 ：负压封闭引流, 烧伤脓毒症, C57BL/6J小鼠, 炎性反应

Abstract：Objective To investigate the effect of vaccum sealing drainage (VSD) on C57BL/6J mice with burn sepsis. Methods A total of 300 male C57BL/6J mice were used to establish the model of burn sepsis. Eighty burned sepsis mice successfully were divided into experimental group and control group according to random number table, 40 mice in each group. In the experimental group, the wound was disposed and treated with VSD, the negative pressure apparatus was connected, and the negative pressure was adjusted to -120 mmHg (1 mmHg=0.133 kPa). In the control group, only wound treatment was performed, and dressing was replaced every 24 h. The fluorescence intensity and blood flow of wound skin, the levels of inflammatory cytokines such as IL-1β, IL-6, IL-8, IL-10, TNF-α and so on were statistically calculated in the two groups, and the cumulative survival rate of 7 d was observed. Results Before treatment, the fluorescence intensity of wound was similar between the two groups, and the difference was not statistically significant (P > 0.05). Compared with before treatment, the fluorescence intensity of the control group increased significantly after 24 h of treatment (P < 0.05), while the fluorescence intensity of the experimental group decreased compared with before treatment (P < 0.05). The difference between the two groups was statistically significant (P < 0.05). Compared with before treatment, the control group showed a decrease on the 1st and 2nd day after treatment, and an increase on the 3rd, 5th and 7th day after treatment, there were statistically significant differences (P < 0.05 or P < 0.01). The blood flow to the wounds on the back of the experimental group gradually increased and were significantly higher than those of the control group after treatment (P < 0.05 or P < 0.01). In the experimental group, the contents of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α were significantly reduced and IL-8 was significantly increased compared with the control group (all P < 0.05), while the changes of IL-10 were not significant (all P > 0.05). From day 2, the survival rates of the experimental group were significantly higher than those of the control group, with statistically significant differences (all P < 0.05). Conclusion VSD treatment for C57BL/6J mice with burn sepsis can reduce bacteria content of wounds, improve blood supply, reduce systemic inflammatory response level of sepsis and improve survival rate.

Key words： Vaccum sealing drainage Burn sepsis C57BL/6J mice Inflammatory response

基金资助:国家自然科学基金资助项目（81501666）。

通讯作者: ▲通讯作者

引用本文:

刘洋白晓智李小强陈子阅何亭▲. 负压封闭引流治疗C57BL/6J烧伤脓毒症小鼠的效果[J]. 中国医药导报, 2018, 15(32): 4-8.
LIU Yang BAI Xiaozhi LI Xiaoqiang CHEN Ziyue HE Ting▲. Effect of vaccum sealing drainage in C57BL/6J mice with burn sepsis. 中国医药导报, 2018, 15(32): 4-8.

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