茵陈蒿汤联合加味大柴胡汤对肝缺血再灌注损伤大鼠的影响

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摘要目的观察茵陈蒿汤联合加味大柴胡汤对大鼠肝缺血再灌注损伤的保护作用。方法将肝脏缺血再灌注模型大鼠按照随机数字表法随机分为缺血再灌注中药组（TCM组，n = 75）、缺血再灌注对照组（Control组，n = 75）和假手术组（Sham组，n = 75）。采用酶联免疫吸附测定（ELISA）法和Western blot法分别检测大鼠血清中白细胞介素-1（IL-1）和肝匀浆组织中细胞间黏附分子-1（ICAM-1）及核转录因子-κB（NF-κB）的含量。结果三组大鼠缺血再灌注后，随着时间的延长，血清IL-1含量逐渐升高，在缺血再灌注后相同时间内，TCM组血清IL-1含量均低于Control组，差异有高度统计学意义（P ＜ 0.01）。随着时间的延长，TCM组及Control组大鼠术后肝匀浆组织NF-κB及ICAM-1的表达逐渐增多，且TCM组各时间点NF-κB和ICAM-1的表达均低于对照组（P ＜ 0.05）。结论茵陈蒿汤联合加味大柴胡汤可明显减轻大鼠肝缺血再灌注的损伤，其可能是通过抑制NF-κB的激活，减少ICAM-1和IL-1的表达，从而在肝脏缺血再灌注损伤中起到保护作用。

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	张北平1 翟淑萍2 苑伟2 颜勇2 章小兵1 王百林2▲

关键词 ：肝缺血再灌注, 白细胞介素-1, 细胞间黏附分子-1, 核转录因子-&kappa, B

Abstract：Objective To observe the protective effect of Major Capillaries Decoction and Bubleurum Decoction on liver ischemia-reperfusion injury in rats. Methods Liver ischemia-reperfusion model rats were randomly divided into ischemia-reperfusion Chinese medicine group (TCM group, n = 75), ischemia-reperfusion control group (control group, n = 75), and sham operation group (sham group, n = 75). The contents of interleukin-1 (IL-1) in serum, intercellular adhesion molecule-1 (ICAM-1) and nuclear factor-kappa B (NF-κB) in rat liver homogenate tissue were measured by enzyme-linked immunosorbent assay (ELISA) and Western blot. Results After ischemia and reperfusion in three groups, serum IL-1 content gradually increased with the extension of time. In the same time after ischemia-reperfusion, the serum IL-1 content of TCM group was significantly lower than that of the control group, the differences were highly statistically significant (P ＜ 0.01). The expression of NF-κB and ICAM-1 in the liver homogenate tissue of TCM group and control group increased gradually with the extension of time, and the expression of TCM group was higher than that of control group at the same time point（P ＜ 0.05）. Conclusion Major Capillaries Decoction and Bubleurum Decoction can significantly alleviate liver ischemia-reperfusion injury in rats. It may be through inhibiting the activation of NF-κB and reducing the expression of ICAM-1 and IL-1 to play a protective role in ischemia-reperfusion injury.

Key words： Hepatic ischemia-reperfusion Interleukin-1 Intercellular adhesion molecule-1 Nuclear factor-κB

基金资助: 广东省自然科学基金项目（2015A030313364）。

通讯作者: ▲通讯作者

引用本文:

张北平1 翟淑萍2 苑伟2 颜勇2 章小兵1 王百林2▲. 茵陈蒿汤联合加味大柴胡汤对肝缺血再灌注损伤大鼠的影响[J]. 中国医药导报, 2018, 15(30): 15-18.
ZHANG Beiping1 ZHAI Shuping2 YUAN Wei2 YAN Yong2 ZHANG Xiaobing1 WANG Bailin2▲. The effect of Major Capillaries Decoction and Bubleurum Decoction on liver ischemia reperfusion injury in rats. 中国医药导报, 2018, 15(30): 15-18.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2018/V15/I30/15

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