Analysis on the quality of loquat leaves from Tangxi
MA Zhelong1 WU Zengyan1 JIANG Fusheng2 DING Zhishan3
1.Department of TCM Pharmacy, the Fifth People′s Hospital of Yuhang District in Hangzhou City, Zhejiang Province, Hangzhou 311100, China;
2.College of Life Science, Zhejiang Chinese Medical University, Zhejiang Province, Hangzhou 310053, China;
3.College of Medical Technology, Zhejiang Chinese Medical University, Zhejiang Province, Hangzhou 310053, China
Abstract：Objective To evaluate the quality of different varieties of loquat leaves from Tangxi. Methods Reference to the 2015 edition of Chinese Pharmacopoeia, the water content, total ash content and extract content of different varieties of loquat leaves were measured by oven drying method, ignition method and hot-dip method. The oleanolic acid and ursolic acid were detected useing Kromasil C18 (250 mm×4.6 mm, 5 μm) column, with mobile phase being acetonitrile-methanol-0.5% ammonium acetate (67∶12∶21, v/v/v), column temperature being at 25℃, flow velocity being 1.0 mL/min, and detection wavelength being at 210 nm. The quercetin and kaempferol were detected by Kromasil C18（250 mm×4.6 mm,5 μm）column, with mobile phase being methanol-0.4% phosphoric acid (50∶50, v/v), column temperature being at 30℃, flow velocity being 1.0 mL/min, and detection wavelength being at 360 nm. Results The range of water content, total ash content, and extract content of different varieties of loquat leaves were 7.87%-9.45%, 5.14%-6.22% and 25.41%-29.68%, respectively, the range of total content of oleanolic acid and ursolic acid was 1.04%-1.25%, all in line with the 2015 edition of Chinese Pharmacopoeia. In addition, the range of total content of quercetin and kaempferol in loquat leaves was 0.17%-0.29%. Conclusion The different varieties of loquat leaves in Tangxi all have good quality and deserve further development and utilization.
2018, Vol. 15 
京公网安备 11010502046598号