Abstract：Objective To establish high-performance liquid chromatography (HPLC) method for the content determination of buddleoside and ginsenoside Rg1 in Zhixue Yangyuan Oral Liquid. Methods HPLC was used to determine the content of buddleoside in Herba Cirsii and ginsenoside Rg1 in Radix Ginseng. The content of buddleoside in Herba Cirsii was determinated with mobile phase consisted of methanol-0.5% acetic acid water (50∶50). The detection wavelength was set at 326 nm. The content of ginsenoside Rg1 in Radix Ginseng was determinated with mobile phase consisted of acetonitrile-water (20∶80). The detection wavelength was set at 203 nm. Results There was a good linear relationship between buddleoside and peak area in the range of 5-160 μg/mL (r = 0.9997) with an average recovery of 97.73%, RSD = 2.01% (n = 6). There was a good linear relationship between ginsenoside Rg1 and peak area in the range of 0.025-0.8 mg/mL (r = 0.9998) with an average recovery of 98.62%, RSD = 2.48% (n = 6). The content determination results of the three batches of samples all met the requirements. Conclusion The method is simple, accurate and reproducible. It is effective in controlling the quality of Zhixue Yangyuan Oral Liquid.
2018, Vol. 15 
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